ArticleApplied and environmental microbiology2010
Maximizing capture efficiency and specificity of magnetic separation for Mycobacterium avium subsp. paratuberculosis cells.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 83 citations in OpenAlex.
- Comparison of Immunomagnetic Bead Separation-Immunosensor Detection and Nested-PCR Methods for Detecting Mycobacterium avium Subspecies paratuberculosis in Cattle Feces.Journal of clinical laboratory analysis · 2025Article
- Evaluation of the reproducibility and performance characteristics of the Phagomagnetic separation-qPCR assay for rapidly detecting viableFrontiers in veterinary science · 2025Article
- Mycobacteriophages in diagnosis and alternative treatment of mycobacterial infections.Frontiers in microbiology · 2023Review
- Are Reactive Oxygen Species (ROS) the Main Mechanism by Which Copper Ion Treatment Degrades the DNA ofMicroorganisms · 2022Article
- Specific Isolation ofInternational journal of molecular sciences · 2022Article
- A Review on Mycobacteriophages: From Classification to Applications.Pathogens (Basel, Switzerland) · 2022Review
- The efficacy and safety of high-pressure processing of food.EFSA journal. European Food Safety Authority · 2022Article
- A rapid phage assay for detection of viable Mycobacterium avium subsp. paratuberculosis in milk.Scientific reports · 2022Article
- IsoMAG-An Automated System for the Immunomagnetic Isolation of Squamous Cell Carcinoma-Derived Circulating Tumor Cells.Diagnostics (Basel, Switzerland) · 2021Article
- Analytical methods of antibody surface coverage and orientation on bio-functionalized magnetic beads: application to immunocapture of TNF-α.Analytical and bioanalytical chemistry · 2021Article
- Phage Amplification Assay for Detection of Mycobacterial Infection: A Review.Microorganisms · 2021Review
- Bacteriophage-Based Methods for Detection of ViableFrontiers in veterinary science · 2021Review
- Detection ofFrontiers in veterinary science · 2021Article
- Efficiency of DNA Isolation Methods Based on Silica Columns and Magnetic Separation Tested for the Detection ofMaterials (Basel, Switzerland) · 2020Article
- A novel one-day phage-based test for rapid detection and enumeration of viable Mycobacterium avium subsp. paratuberculosis in cows' milk.Applied microbiology and biotechnology · 2020Article
- Human Liver Microsomes Immobilized on Magnetizable Beads: A Novel Approach to Study In Vitro Drug Metabolism.Drug metabolism and disposition: the biological fate of chemicals · 2020Article
- Is the transmission of Mycobacterium avium subspecies paratuberculosis (MAP) infection through milk intended to feed calves an overlooked item in paratuberculosis control programs?Tropical animal health and production · 2020Article
- Estimation of Performance Characteristics of Analytical Methods forFrontiers in microbiology · 2019Article
- Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis.Biosensors · 2018Article
- Surface-Enhanced Raman Scattering Nanoparticles for Multiplexed Imaging of Bladder Cancer Tissue Permeability and Molecular Phenotype.ACS nano · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to introduce specificity for Mycobacterium avium subsp. paratuberculosis prior to a phage amplification assay, various magnetic-separation approaches, involving either antibodies or peptides, were evaluated in terms of the efficiency of capture (expressed as a percentage) of M. avium subsp. paratuberculosis cells and the percentage of nonspecific binding by other Mycobacterium spp. A 50:50 mixture of MyOne Tosylactivated Dynabeads coated with the chemically synthesized M. avium subsp. paratuberculosis-specific peptides biotinylated aMp3 and biotinylated aMptD (i.e., peptide-mediated magnetic separation [PMS]) proved to be the best magnetic-separation approach for achieving 85 to 100% capture of M. avium subsp. paratuberculosis and minimal (<1%) nonspecific recovery of other Mycobacterium spp. (particularly if beads were blocked with 1% skim milk before use) from broth samples containing 10(3) to 10(4) CFU/ml. When PMS was coupled with a recently optimized phage amplification assay and used to detect M. avium subsp. paratuberculosis in 50-ml volumes of spiked milk, the mean 50% limit of detection (LOD(50)) was 14.4 PFU/50 ml of milk (equivalent to 0.3 PFU/ml). This PMS-phage assay represents a novel, rapid method for the detection and enumeration of viable M. avium subsp. paratuberculosis organisms in milk, and potentially other sample matrices, with results available within 48 h.
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