ArticleJournal of pharmacological and toxicological methods
Sampling port for real-time analysis of bioaerosol in whole body exposure system for animal aerosol model development.
Article in Journal of pharmacological and toxicological methods. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Targeted dose delivery of Mycobacterium tuberculosis in mice using silicon antifoaming agent via aerosol exposure system.PloS one · 2022Article
- A Vibrating Mesh Nebulizer as an Alternative to the Collison Three-Jet Nebulizer for Infectious Disease Aerobiology.Applied and environmental microbiology · 2019Article
- Evaluation of Exposure toJournal of aerosol science · 2017Article
- Bioaerosol sampling: sampling mechanisms, bioefficiency and field studies.The Journal of hospital infection · 2016Review
- CLARITY and PACT-based imaging of adult zebrafish and mouse for whole-animal analysis of infections.Disease models & mechanisms · 2015Article
- Article
- Impact of surfactant protein D, interleukin-5, and eosinophilia on Cryptococcosis.Infection and immunity · 2014Article
- ABSL-4 aerobiology biosafety and technology at the NIH/NIAID integrated research facility at Fort Detrick.Viruses · 2014Article
- Reduced virulence of an extensively drug-resistant outbreak strain of Mycobacterium tuberculosis in a murine model.PloS one · 2014Article
- Article
- Ultra-low dose of Mycobacterium tuberculosis aerosol creates partial infection in mice.Tuberculosis (Edinburgh, Scotland) · 2012Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
introductionMultiple factors influence the viability of aerosolized bacteria. The delivery of aerosols is affected by chamber conditions (humidity, temperature, and pressure) and bioaerosol characteristics (particle number, particle size distribution, and viable aerosol concentration). Measurement of viable aerosol concentration and particle size is essential to optimize viability and lung delivery. The Madison chamber is widely used to expose small animals to infectious aerosols.
methodsA multiplex sampling port was added to the Madison chamber to measure the chamber conditions and bioaerosol characteristics. Aerosols of three pathogens (Bacillus anthracis, Yersinia pestis, and Mycobacterium tuberculosis) were generated under constant conditions and their bioaerosol characteristics were analyzed. Airborne microbes were captured using an impinger or BioSampler. The particle size distribution of airborne microbes was determined using an aerodynamic particle sizer (APS). Viable aerosol concentration, spray factor (viable aerosol concentration/inoculum concentration), and dose presented to the mouse were calculated. Dose retention efficiency and viable aerosol retention rate were calculated from the sampler titers to determine the efficiency of microbe retention in lungs of mice.
resultsB. anthracis, Y. pestis, and M. tuberculosis aerosols were sampled through the port. The count mean aerodynamic sizes were 0.98, 0.77, and 0.78 μm with geometric standard deviations of 1.60, 1.90, and 2.37, and viable aerosol concentrations in the chamber were 211, 57, and 1 colony-forming unit (CFU)/mL, respectively. Based on the aerosol concentrations, the doses presented to mice for the three pathogens were 2.5e5, 2.2e4 and 464 CFU. DISCUSSION: Using the multiplex sampling port we determined whether the animals were challenged with an optimum bioaerosol based on dose presented and respirable particle size.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.