ArticleMicrobial cell factories2010
Isolation of cell-free bacterial inclusion bodies.
Article in Microbial cell factories, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed.
- Potential of inclusion bodies for disease control in aquaculture and livestock.Veterinary and animal science · 2026Review
- Expression, Solubilization, and Refolding Recovery of a Novel l‑Asparaginase-Arginase 1 Chimera fromACS omega · 2026Article
- Expression of a Tuberculosis-Associated Immunogenic Protein inLife (Basel, Switzerland) · 2025Article
- Engineering Human Cells Expressing CRISPR/Cas9-Synergistic Activation Mediators for Recombinant Protein Production.International journal of molecular sciences · 2023Article
- Catalytically-active inclusion bodies for biotechnology-general concepts, optimization, and application.Applied microbiology and biotechnology · 2020Review
- Nucleic acids in inclusion bodies obtained from E. coli cells expressing human interferon-gamma.Microbial cell factories · 2020Article
- Coiled-coil inspired functional inclusion bodies.Microbial cell factories · 2020Article
- Improvement of solubility and yield of recombinant protein expression inResearch in pharmaceutical sciences · 2019Article
- Spontaneous C-cleavage of a truncated intein as fusion tag to produce tag-free VP1 inclusion body nanoparticle vaccine against CVB3-induced viral myocarditis by the oral route.Microbial cell factories · 2019Article
- Effect of buffer additives on solubilization and refolding of reteplase inclusion bodies.Research in pharmaceutical sciences · 2018Article
- Custom made inclusion bodies: impact of classical process parameters and physiological parameters on inclusion body quality attributes.Microbial cell factories · 2018Article
- Catalytically active inclusion bodies of L-lysine decarboxylase from E. coli for 1,5-diaminopentane production.Scientific reports · 2018Article
- Process for production and purification of Lethal Toxin Neutralizing Factor (LTNF) fromJournal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986) · 2018Article
- ESCRT-mediated vesicle concatenation in plant endosomes.The Journal of cell biology · 2017Article
- Direct Conversion of an Enzyme from Native-like to Amyloid-like Aggregates within Inclusion Bodies.Biophysical journal · 2017Article
- Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics.BioMed research international · 2017Review
- Bacterial mimetics of endocrine secretory granules as immobilized in vivo depots for functional protein drugs.Scientific reports · 2016Article
- Functional inclusion bodies produced in the yeast Pichia pastoris.Microbial cell factories · 2016Article
- Enhanced production of polyhydroxybutyrate by multiple dividing E. coli.Microbial cell factories · 2016Article
- First principles design of a core bioenergetic transmembrane electron-transfer protein.Biochimica et biophysica acta · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBacterial inclusion bodies are submicron protein clusters usually found in recombinant bacteria that have been traditionally considered as undesirable products from protein production processes. However, being fully biocompatible, they have been recently characterized as nanoparticulate inert materials useful as scaffolds for tissue engineering, with potentially wider applicability in biomedicine and material sciences. Current protocols for inclusion body isolation from Escherichia coli usually offer between 95 to 99% of protein recovery, what in practical terms, might imply extensive bacterial cell contamination, not compatible with the use of inclusion bodies in biological interfaces.
resultsUsing an appropriate combination of chemical and mechanical cell disruption methods we have established a convenient procedure for the recovery of bacterial inclusion bodies with undetectable levels of viable cell contamination, below 10⁻¹ cfu/ml, keeping the particulate organization of these aggregates regarding size and protein folding features.
conclusionsThe application of the developed protocol allows obtaining bacterial free inclusion bodies suitable for use in mammalian cell cultures and other biological interfaces.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.