ArticleParasites & vectors2010
Analysis and characterization of the genes encoding the Dicer and Argonaute proteins of Schistosoma japonicum.
Article in Parasites & vectors, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Multifunctional Roles of MicroRNAs in Schistosomiasis.Frontiers in microbiology · 2022Review
- MicroRNAs Are Involved in the Regulation of Ovary Development in the Pathogenic Blood Fluke Schistosoma japonicum.PLoS pathogens · 2016Article
- High-throughput sequencing of RNAs isolated by cross-linking immunoprecipitation (HITS-CLIP) reveals Argonaute-associated microRNAs and targets in Schistosoma japonicum.Parasites & vectors · 2015Article
- Role of microRNAs in schistosomes and schistosomiasis.Frontiers in cellular and infection microbiology · 2014Review
- A deep analysis of the small non-coding RNA population in Schistosoma japonicum eggs.PloS one · 2013Article
- Chromatin structural changes around satellite repeats on the female sex chromosome in Schistosoma mansoni and their possible role in sex chromosome emergence.Genome biology · 2012Article
- Identification and characterization of argonaute protein, Ago2 and its associated small RNAs in Schistosoma japonicum.PLoS neglected tropical diseases · 2012Article
- Schistosoma comparative genomics: integrating genome structure, parasite biology and anthelmintic discovery.Trends in parasitology · 2011Review
- Insights into the functional biology of schistosomes.Parasites & vectors · 2011Article
- Differential gene expression in Schistosoma japonicum schistosomula from Wistar rats and BALB/c mice.Parasites & vectors · 2011Article
- Comparative Analysis ofIranian journal of parasitologyArticle
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Authors and funding
6 authors.
Funding
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Abstract
backgroundThe Dicer and Argonaute(AGO) proteins within the small RNA regulatory pathways (SRRPs) play an indispensable role in regulation of gene expression. In this study, we analyzed two genes, Dicer and Argonaute, from Schistosoma japonicum, along with their expression through a combination of bioinformatics and experimental approaches.
resultsOur results indicate that one Dicer and four Argonaute genes exist in Schistosoma japonicum, termed SjDicer and SjAGO1, 2, 3, and 4, respectively. SjDicer encodes 2590 amino acid residues that contains 5 conserved domains, including one amino-terminal helicase domain, one PAZ (Piwi-Argonaut-Zwille) domain, two RNAse III domains, and one dsRNA-binding domain. SjAGO1, 2, and 3 encode 1009, 945, and 904 amino acid residues, respectively, all of which contain PAZ and PIWI domains. In addition, we analyzed the expression profiles of SjDicer and SjAGO1 genes by qRT-PCR in eggs, miracidium, cercariae, schistosomula, and adult worms. Results showed consistent expression of both SjDicer and SjAGO1 in different stages; however, their expression levels were stage-dependent, with the highest being in the miracidium stage.
conclusionsThis study provided the sequence of the Dicer and Ago genes of S. japonicum and their expression profiles which are essential for further investigation of functions of miRNA in Schistosoma japonicum.
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