ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2011
Dissecting chromatin interactions in living cells from protein mobility maps.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Article
- Article
- Measuring Mobility in Chromatin by Intensity-Sorted FCS.Biophysical journal · 2019Article
- Multimodal Light Microscopy Approaches to Reveal Structural and Functional Properties of Promyelocytic Leukemia Nuclear Bodies.Frontiers in oncology · 2018Review
- On the way of revealing coactivator complexes cross-talk during transcriptional activation.Cell & bioscience · 2016Review
- Review
- BRCA2 diffuses as oligomeric clusters with RAD51 and changes mobility after DNA damage in live cells.The Journal of cell biology · 2014Article
- Specificity, propagation, and memory of pericentric heterochromatin.Molecular systems biology · 2014Article
- Retrieving the intracellular topology from multi-scale protein mobility mapping in living cells.Nature communications · 2014Article
- Chromatin dynamics during DNA repair revealed by pair correlation analysis of molecular flow in the nucleus.Biophysical journal · 2014Article
- Chromatin targeting signals, nucleosome positioning mechanism and non-coding RNA-mediated regulation of the chromatin remodeling complex NoRC.PLoS genetics · 2014Article
- Advanced fluorescence microscopy methods for the real-time study of transcription and chromatin dynamics.Transcription · 2014Review
- Quantifying transient binding of ISWI chromatin remodelers in living cells by pixel-wise photobleaching profile evolution analysis.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Monitoring dynamic binding of chromatin proteins in vivo by fluorescence correlation spectroscopy and temporal image correlation spectroscopy.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Bridging the resolution gap in structural modeling of 3D genome organization.PLoS computational biology · 2011Review
- Dynamic as well as stable protein interactions contribute to genome function and maintenance.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
- Nuclear proteins: finding and binding target sites in chromatin.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
- Binding kinetics of human ISWI chromatin-remodelers to DNA repair sites elucidate their target location mechanism.Nucleus (Austin, Tex.)Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genome of eukaryotes is organized into a dynamic nucleoprotein complex referred to as chromatin, which can adopt different functional states. Both the DNA and the protein component of chromatin are subject to various post-translational modifications that define the cell's gene expression program. Their readout and establishment occurs in a spatio-temporally coordinated manner that is controlled by numerous chromatin-interacting proteins. Binding to chromatin in living cells can be measured by a spatially resolved analysis of protein mobility using fluorescence microscopy based approaches. Recent advancements in the acquisition of protein mobility data using fluorescence bleaching and correlation methods provide data sets on diffusion coefficients, binding kinetics, and cellular concentrations on different time and length scales. The combination of different techniques is needed to dissect the complex interplay of diffusive translocations, binding events, and mobility constraints of the chromatin environment. While bleaching techniques have their strength in the characterization of particles that are immobile on the second/minute time scale, a correlation analysis is advantageous to characterize transient binding events with millisecond residence time. The application and synergy effects of the different approaches to obtain protein mobility and interaction maps in the nucleus are illustrated for the analysis of heterochromatin protein 1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.