ArticleThe Journal of molecular diagnostics : JMD2010
Genotyping of DNA samples isolated from formalin-fixed paraffin-embedded tissues using preamplification.
Article in The Journal of molecular diagnostics : JMD, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- The variant T allele of PvuII in ESR1 gene is a prognostic marker in early breast cancer survival.Scientific reports · 2021Trial
- Insulin-like growth factor 1 receptor expression and IGF1R 3129G > T polymorphism are associated with response to neoadjuvant chemotherapy in breast cancer patients: results from the NEOZOTAC trial (BOOG 2010-01).Breast cancer research : BCR · 2016Trial
- Associations between APOL1 genetic variants and blood pressure in African American mothers and children from a U.S. pregnancy cohort: Modification by air pollution exposures.Environmental research · 2022Article
- Rapid and sensitive detection of SARS-CoV-2 variants in nasopharyngeal swabs and wastewaters.Diagnostic microbiology and infectious disease · 2022Article
- A novel approach for rapid cell assessment to estimate DNA recovery from human bone tissue.Forensic science, medicine, and pathology · 2021Article
- Genetic polymorphisms in ABCG2 and CYP1A2 are associated with imatinib dose reduction in patients treated for gastrointestinal stromal tumors.The pharmacogenomics journal · 2019Article
- Genetic identification of endoscopic biopsies after unnecessary gastrectomy: Case report and medico-legal evaluation.International journal of surgery case reports · 2019Article
- Fetal-Not Maternal-APOL1 Genotype Associated with Risk for Preeclampsia in Those with African Ancestry.American journal of human genetics · 2018Article
- Genetic polymorphisms as predictive biomarker of survival in patients with gastrointestinal stromal tumors treated with sunitinib.The pharmacogenomics journal · 2018Article
- HSD3B1 and resistance to androgen-deprivation therapy in prostate cancer: a retrospective, multicohort study.The Lancet. Oncology · 2016Article
- Genome-Wide Association Studies for Taxane-Induced Peripheral Neuropathy in ECOG-5103 and ECOG-1199.Clinical cancer research : an official journal of the American Association for Cancer Research · 2015Article
- Genotyping concordance in DNA extracted from formalin-fixed paraffin embedded (FFPE) breast tumor and whole blood for pharmacogenetic analyses.Molecular oncology · 2015Article
- Discrepancies between VEGF -1154 G>A polymorphism analysis performed in peripheral blood samples and FFPE tissue.International journal of molecular sciences · 2014Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA isolated from formalin-fixed paraffin-embedded (FFPE) tissue is often fragmented and cross-linked and is therefore difficult to genotype. To enable this source of DNA for genotyping analysis using Taqman probes, we tested whether enrichment of the target genes would increase the amount of available DNA. For enrichment of the target genes, we used preamplification by means of diluted Taqman assays. To establish the appropriateness of preamplification, we used DNA extracted from paraffin-embedded tissue and compared the genotyping results of a series of single nucleotide polymorphisms assessed in DNA samples with and without preamplification. In a subset of patients, DNA was isolated from both blood and FFPE tissue to test the reliability of genotyping results derived after preamplification. We found an increase in call rate after preamplification and a convincing concordance in genotype. Based on our findings, we can safely conclude that preamplification of DNA isolated from paraffin-embedded tissue is a valuable and reliable method to optimize genotyping results.
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