ArticleThe Journal of biological chemistry2010
Modulation of energy transfer pathways between mitochondria and myofibrils by changes in performance of perfused heart.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- CardiacHeart failure reviews · 2023Review
- Rat and mouse cardiomyocytes show subtle differences in creatine kinase expression and compartmentalization.PloS one · 2023Article
- Ontogeny of cardiomyocytes: ultrastructure optimization to meet the demand for tight communication in excitation-contraction coupling and energy transfer.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Article
- Matrix revisited: mechanisms linking energy substrate metabolism to the function of the heart.Circulation research · 2014Review
- The location of energetic compartments affects energetic communication in cardiomyocytes.Frontiers in physiology · 2014Article
- Unchanged mitochondrial organization and compartmentation of high-energy phosphates in creatine-deficient GAMT-/- mouse hearts.American journal of physiology. Heart and circulatory physiology · 2013Article
- Molecular dynamics simulations of creatine kinase and adenine nucleotide translocase in mitochondrial membrane patch.The Journal of biological chemistry · 2012Article
- Analysis of molecular movement reveals latticelike obstructions to diffusion in heart muscle cells.Biophysical journal · 2012Article
- Sensitivity analysis of flux determination in heart by H₂ ¹⁸O -provided labeling using a dynamic Isotopologue model of energy transfer pathways.PLoS computational biology · 2012Article
- Permeabilized rat cardiomyocyte response demonstrates intracellular origin of diffusion obstacles.Biophysical journal · 2011Article
- Analyzing the functional properties of the creatine kinase system with multiscale 'sloppy' modeling.PLoS computational biology · 2011Article
- Symbolic flux analysis for genome-scale metabolic networks.BMC systems biology · 2011Article
- Molecular system bioenergics of the heart: experimental studies of metabolic compartmentation and energy fluxes versus computer modeling.International journal of molecular sciences · 2011Review
- Where have the fluxes gone?The Journal of biological chemistry · 2010Article
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6 authors.
Funding
Abstract
In the heart, the energy supplied by mitochondria to myofibrils is continuously and finely tuned to the contraction requirement over a wide range of cardiac loads. This process is mediated both by the creatine kinase (CK) shuttle and by direct ATP transfer. The aim of this study was to identify the contribution of energy transfer pathways at different cardiac performance levels. For this, five protocols of (31)P NMR inversion and saturation transfer experiments were performed at different performance levels on Langendorff perfused rat hearts. The cardiac performance was changed either through variation of external calcium in the presence or absence of isoprenaline or through variation of LV balloon inflation. The recordings were analyzed by mathematical models composed on the basis of different energy transfer pathway configurations. According to our results, the total CK unidirectional flux was relatively stable when the cardiac performance was changed by increasing the calcium concentration or variation of LV balloon volume. The stability of total CK unidirectional flux is lost at extreme energy demand levels leading to a rise in inorganic phosphate, a drop of ATP and phosphocreatine, a drop of total CK unidirectional flux, and to a bypass of CK shuttle by direct ATP transfer. Our results provide experimental evidence for the existence of two pathways of energy transfer, direct ATP transfer, and PCr transfer through the CK shuttle, whose contribution may vary depending on the metabolic status of the heart.
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