ArticleVirology journal2010
Production, purification and characterization of polyclonal antibody against the truncated gK of the duck enteritis virus.
Article in Virology journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 9 citations in OpenAlex.
- Review
- Two nuclear localization signals regulate intracellular localization of the duck enteritis virus UL13 protein.Poultry science · 2021Article
- US10 Protein Is Crucial but not Indispensable for Duck Enteritis Virus Infection in Vitro.Scientific reports · 2018Article
- Molecular characterization of duck enteritis virus UL41 protein.Virology journal · 2018Article
- Characterization of the duck enteritis virus UL55 protein.Virology journal · 2011Article
- Characterization of duck enteritis virus UL53 gene and glycoprotein K.Virology journal · 2011Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Duck virus enteritis (DVE) is an acute, contagious herpesvirus infection of ducks, geese, and swans, which has produced significant economic losses in domestic and wild waterfowl. With the purpose of decreasing economic losses in the commercial duck industry, studying the unknown glycoprotein K (gK) of DEV may be a new method for preferably preventing and curing this disease. So this is the first time to product and purify the rabbit anti-tgK polyclonal antibody. Through the western blot and ELISA assay, the truncated glycoprotein K (tgK) has good antigenicity, also the antibody possesses high specificity and affinity. Meanwhile the rabbit anti-tgK polyclonal antibody has the potential to produce subunit vaccines and the functions of neutralizing DEV and anti-DEV infection because of its neutralization titer. Indirect immunofluorescent microscopy using the purified rabbit anti-tgK polyclonal antibody as diagnostic antibody was susceptive to detect a small quantity of antigen in tissues or cells. This approach also provides effective experimental technology for epidemiological investigation and retrospective diagnose of the preservative paraffin blocks.
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Registered trials
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