ArticleACS chemical biology2011
A p38α-selective chemosensor for use in unfractionated cell lysates.
Article in ACS chemical biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Let the peptides shine: SOX (Sulfonamido-OXine)-labelled peptides for direct kinase and phosphatase monitoring.RSC chemical biology · 2026Review
- A Constellation of Fluorescent Biosensors to Illuminate the Galaxy of Protein Kinases.Chembiochem : a European journal of chemical biology · 2026Review
- Advances in Activity-Based Sensing Probes for Isoform-Selective Imaging of Enzymatic Activity.Angewandte Chemie (International ed. in English) · 2021Review
- Quantification of ERK Kinase Activity in Biological Samples Using Differential Sensing.ACS chemical biology · 2020Article
- Safety profiling of genetically engineered Pim-1 kinase overexpression for oncogenicity risk in human c-kit+ cardiac interstitial cells.Gene therapy · 2019Article
- Quantification of Cell Signaling Networks Using Kinase Activity Chemosensors.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Temporal Analysis of PP2A Phosphatase Activity During Insulin Stimulation Using a Direct Activity Probe.ACS chemical biology · 2016Article
- Fluorescent Reporters and Biosensors for Probing the Dynamic Behavior of Protein Kinases.Proteomes · 2015Review
- Quantification of a Pharmacodynamic ERK End Point in Melanoma Cell Lysates: Toward Personalized Precision Medicine.ACS medicinal chemistry letters · 2015Article
- Differential sensing of MAP kinases using SOX-peptides.Angewandte Chemie (International ed. in English) · 2014Article
- Selective mitogen activated protein kinase activity sensors through the application of directionally programmable D domain motifs.Biochemistry · 2014Article
- Quantification of protein kinase enzymatic activity in unfractionated cell lysates using CSox-based sensors.Current protocols in chemical biology · 2014Article
- Detecting kinase activities from single cell lysate using concentration-enhanced mobility shift assay.Analytical chemistry · 2014Article
- Microfluidic probe for single-cell analysis in adherent tissue culture.Nature communications · 2014Article
- Proteogenomic convergence for understanding cancer pathways and networks.Clinical proteomics · 2014Review
- Interrogating signaling nodes involved in cellular transformations using kinase activity probes.Chemistry & biology · 2012Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Recent efforts have identified the p38α Ser/Thr kinase as a potential target for the treatment of inflammatory diseases as well as non-small cell lung carcinoma. Despite the significance of p38α, no direct activity probe compatible with cell lysate analysis exists. Instead, proxies for kinase activation, such as phosphospecific antibodies, which do not distinguish between p38 isoforms, are often used. Our laboratory has recently developed a sulfonamido-oxine (Sox) fluorophore that undergoes a significant increase in fluorescence in response to phosphorylation at a proximal residue, allowing for real-time activity measurements. Herein we report the rational design of a p38α-selective chemosensor using this approach. We have validated the selectivity of this sensor using specific inhibitors and immunodepletions and show that p38α activity can be monitored in crude lysates from a variety of cell lines, allowing for the potential use of this sensor in both clinical and basic science research applications.
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Registered trials
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