ArticleMolecular biology reports2011
The characterization of transaldolase gene tal from Pichia stipitis and its heterologous expression in Fusarium oxysporum.
Article in Molecular biology reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed, 15 citations in OpenAlex.
- Recent Progress on Systems and Synthetic Biology Approaches to Engineer Fungi As Microbial Cell Factories.Current genomics · 2016Article
- Metabolic Engineering of Fusarium oxysporum to Improve Its Ethanol-Producing Capability.Frontiers in microbiology · 2016Article
- Enhanced xylose fermentation and ethanol production by engineered Saccharomyces cerevisiae strain.AMB Express · 2015Article
- Constitutive homologous expression of phosphoglucomutase and transaldolase increases the metabolic flux of Fusarium oxysporum.Microbial cell factories · 2014Article
- Challenges in ethanol production with Fusarium oxysporum through consolidated bioprocessing.Bioengineered · 2014Article
- Expression, characterization and 2,4,6-trichlorophenol degradation of laccase from Monilinia fructigena.Molecular biology reports · 2012Article
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Authors and funding
9 authors at 3 institutions in 1 country.
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Abstract
The 972 bp length of transaldolase gene tal was cloned from Pichia stipitis CICC1960, encoding a 323 amino acid protein with a calculated molecular mass of 35.36 kDa and isoelectric point of 5.20. Real time PCR analysis demonstrated that the mRNA transcript level of constitutive tal gene rise on xylose, glucose, fructose, mannose, galactose and sucrose as carbon source, respectively. Furthermore, the transcription of tal gene in P. stipitis on xylose was higher than on other carbon source, indicating that transaldolase plays a part in xylose utilization. To deeply study the tal gene biological function, it was expressed in Fusarium oxysporum CCTCC M209040. Recombinant transaldolase activity of transformant F. oxysporum M209040-Tal2 was about 0.52 U mg(-1) protein and was 1.57 times higher than that of the wild type F. oxysporum CCTCC M209040, indicating that the improvement of transaldolase activity in transformant was due to expression of the exogenous tal gene. Growth of transformant F. oxysporum M209040-Tal2 without selection pressure did not affect the level of hygromycin resistance of the transformants, suggesting that integrated tal gene was stable in mitosis. Fermentation trials of F. oxysporum M209040-Tal2 showed that the ethanol yield improved by 8.39 and 11.71% on glucose and xylose substrates, respectively, demonstrating that the expression of tal gene from P. stipitis CICC1960 in F. oxysporum CCTCC M209040 could improve ethanol production.
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