ArticleImmunogenetics2010
Selection, trans-species polymorphism, and locus identification of major histocompatibility complex class IIβ alleles of New World ranid frogs.
Article in Immunogenetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Characterization of an MHC class II b gene reveals high heterogeneity and polymorphism among populations of non-native bullfrogs with complex invasion histories.Journal of genetics · 2026Article
- The Amphibian Major Histocompatibility Complex-A Review and Future Outlook.Journal of molecular evolution · 2025Review
- Evolution of MHC IIB Diversity Across Cichlid Fish Radiations.Genome biology and evolution · 2023Review
- Spatiotemporal adaptive evolution of an MHC immune gene in a frog-fungus disease system.Heredity · 2021Article
- Cartilaginous fish class II genes reveal unprecedented old allelic lineages and confirm the late evolutionary emergence of DM.Molecular immunology · 2020Article
- Functional variation at an expressed MHC class IIβ locus associates with Ranavirus infection intensity in larval anuran populations.Immunogenetics · 2019Article
- Transcriptome analyses of immune tissues from three Japanese frogs (genus Rana ) reveals their utility in characterizing major histocompatibility complex class II.BMC genomics · 2017Article
- Molecular characterization of MHC class IIB genes of sympatric Neotropical cichlids.BMC genetics · 2017Article
- Molecular characterization of MHC class II in the Australian invasive cane toad reveals multiple splice variants.Immunogenetics · 2016Article
- Adaptive tolerance to a pathogenic fungus drives major histocompatibility complex evolution in natural amphibian populations.Proceedings. Biological sciences · 2016Article
- Susceptibility of amphibians to chytridiomycosis is associated with MHC class II conformation.Proceedings. Biological sciences · 2015Article
- 454 screening of individual MHC variation in an endemic island passerine.Immunogenetics · 2015Article
- Identification and characterization of major histocompatibility complex class IIB alleles from three species of European ranid frogs.Molecular biology research communications · 2014Article
- Article
- Genetic diversity of MHC class I loci in six non-model frogs is shaped by positive selection and gene duplication.Heredity · 2012Article
- Extremely high major histocompatibility complex class IIb gene intron 2 variation and population structure in Chinese alligator.Journal of genetics · 2012Article
- Evidence for evolutionary convergence at MHC in two broadly distributed mesocarnivores.Immunogenetics · 2012Article
- MHC genotypes associate with resistance to a frog-killing fungus.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genes encoded by the major histocompatibility complex (MHC) play key roles in the vertebrate immune system. However, our understanding of the evolutionary processes and underlying genetic mechanisms shaping these genes is limited in many taxa, including amphibians, a group currently impacted by emerging infectious diseases. To further elucidate the evolution of the MHC in frogs (anurans) and develop tools for population genetics, we surveyed allelic diversity of the MHC class II β1 domain in both genomic and complementary DNA of seven New World species in the genus Rana (Lithobates). To assign locus affiliation to our alleles, we used a "gene walking" technique to obtain intron 2 sequences that flanked MHC class IIβ exon 2. Two distinct intron sequences were recovered, suggesting the presence of at least two class IIβ loci in Rana. We designed a primer pair that successfully amplified an orthologous locus from all seven Rana species. In total, we recovered 13 alleles and documented trans-species polymorphism for four of the alleles. We also found quantitative evidence of selection acting on amino acid residues that are putatively involved in peptide binding and structural stability of the β1 domain of anurans. Our results indicated that primer mismatch can result in polymerase chain reaction (PCR) bias, which influences the number of alleles that are recovered. Using a single locus may minimize PCR bias caused by primer mismatch, and the gene walking technique was an effective approach for generating single-copy orthologous markers necessary for future studies of MHC allelic variation in natural amphibian populations.
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