ArticleJournal of clinical microbiology2010
Prevalence and diversity of Borrelia species in ticks that have bitten humans in Sweden.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 49 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
49 citing papers in PubMed, 1 synthesis or guideline pooled it, 106 citations in OpenAlex.
- Laboratory Methods for Detection of Infectious Agents and Serological Response in Humans With Tick-Borne Infections: A Systematic Review of Evaluations Based on Clinical Patient Samples.Frontiers in public health · 2021Pooled it
- Single-step immunoblot tests with recombinant protein antigens for detecting IgG and IgM antibodies in Lyme disease.Microbiology spectrum · 2026Article
- Borrelia valaisiana - a candidate human pathogen? Insights from the tick-borne diseases STING study.BMC infectious diseases · 2026Article
- High-throughput screening for tick-borne pathogens in Ixodid ticks collected through crowdsourcing in northern Sweden.Acta veterinaria Scandinavica · 2026Article
- Investigation of erythema migrans patients identifies Borrelia species and Neoehrlichia mikurensis with implications for clinical assessment.Scientific reports · 2025Observational
- Significance of Detecting Serum Antibodies to Outer Surface Protein A of Lyme DiseaseDiagnostics (Basel, Switzerland) · 2024Article
- Coinfection ofPathogens (Basel, Switzerland) · 2024Review
- Analysis of macerated ticks using Boolean logic gating colorimetric isothermal nucleic acid assays for Lyme Borrelia and Ixodes scapularis ticks.Scientific reports · 2023Article
- The AxBioTick Study:Microorganisms · 2023Article
- Lyme Neuroborreliosis-Significant Local Variations in Incidence within a Highly Endemic Region in Sweden.Microorganisms · 2023Article
- A Novel High Discriminatory Protocol for the Detection ofPathogens (Basel, Switzerland) · 2022Article
- Epizootiological aspects of natural nidality ofVeterinary world · 2022Article
- The raccoon dog (International journal for parasitology. Parasites and wildlife · 2021Article
- Long-term study of Borrelia and Babesia prevalence and co-infection in Ixodes ricinus and Dermacentor recticulatus ticks removed from humans in Poland, 2016-2019.Parasites & vectors · 2021Article
- First Record of a Suspected Human-PathogenicMicroorganisms · 2021Article
- Lyme neuroborreliosis in Swedish children-PCR as a complementary diagnostic method for detection of Borrelia burgdorferi sensu lato in cerebrospinal fluid.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Article
- Migratory birds as disseminators of ticks and the tick-borne pathogens Borrelia bacteria and tick-borne encephalitis (TBE) virus: a seasonal study at Ottenby Bird Observatory in South-eastern Sweden.Parasites & vectors · 2020Article
- Spatial patterns of pathogen prevalence in questing Ixodes ricinus nymphs in southern Scandinavia, 2016.Scientific reports · 2020Article
- Lyme Borreliosis with Scalp Eschar Mimicking Rickettsial Infection, Austria.Emerging infectious diseases · 2020Article
- Human Borrelia miyamotoi Infection, Austria.Emerging infectious diseases · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the genus Borrelia are among the most common infectious agents causing tick-borne disease in humans worldwide. Here, we developed a Light Upon eXtension (LUX) real-time PCR assay that can detect and quantify Borrelia species in ticks that have fed on humans, and we applied the assay to 399 such ticks. Borrelia PCR-positive ticks were identified to species level by sequencing the products of conventional PCR performed using Borrelia group-specific primers. There was a 19% prevalence of Borrelia spp. in the detached ticks, and the number of spirochetes per Borrelia PCR-positive tick ranged from 2.0 × 10(2) to 4.9 × 10(5), with a median of 7.8 × 10(3) spirochetes. Adult ticks had a significantly larger number of spirochetes, with a median of 8.4 × 10(3) compared to the median of nymphs of 4.4 × 10(3). [corrected] Adult ticks also exhibited a higher prevalence of Borrelia (33%) than nymphs (14%). Among the identified species, Borrelia afzelii was found to predominate (61%) and was followed by B. garinii (23%), B. valaisiana (13%), B. burgdorferi sensu stricto (1%), B. lusitaniae (1%), and B. miyamotoi-like (1%). Also, 3% of the ticks were coinfected with multiple strains of B. afzelii. Notably, this is the first report of B. lusitaniae being detected in ticks in Sweden. Our LUX real-time PCR assay proved to be more sensitive than a corresponding TaqMan assay. In conclusion, the novel LUX real-time PCR method is a rapid and sensitive tool for detection and quantification of Borrelia spp. in ticks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.