ArticleMolecular biology of the cell2010
Autocrine transforming growth factor-{beta}1 activation mediated by integrin {alpha}V{beta}3 regulates transcriptional expression of laminin-332 in Madin-Darby canine kidney epithelial cells.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Article
- Density-Dependent Migration Characteristics of Cancer Cells Driven by Pseudopod Interaction.Frontiers in cell and developmental biology · 2022Article
- Regulation of Intestinal Epithelial Barrier and Immune Function by Activated T Cells.Cellular and molecular gastroenterology and hepatology · 2021Article
- Actin-ring segment switching drives nonadhesive gap closure.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Laminins in Epithelial Cell Polarization: Old Questions in Search of New Answers.Cold Spring Harbor perspectives in biology · 2017Review
- Transforming growth factor β1 enhances adhesion of endometrial cells to mesothelium by regulating integrin expression.BMB reports · 2017Article
- Bidirectional signalling between EphA2 and ephrinA1 increases tubular cell attachment, laminin secretion and modulates erythropoietin expression after renal hypoxic injury.Pflugers Archiv : European journal of physiology · 2016Article
- Integrin α3β1 regulates kidney collecting duct development via TRAF6-dependent K63-linked polyubiquitination of Akt.Molecular biology of the cell · 2015Article
- Aberrant expression of laminin-332 promotes cell proliferation and cyst growth in ARPKD.American journal of physiology. Renal physiology · 2014Article
- Resident mesenchymal cells and fibrosis.Biochimica et biophysica acta · 2013Article
- Transforming growth factor β-1 stimulates profibrotic epithelial signaling to activate pericyte-myofibroblast transition in obstructive kidney fibrosis.The American journal of pathology · 2013Article
- TGF-β and renal fibrosis: a Pandora's box of surprises.The American journal of pathology · 2012Article
- Vectorial secretion of CTGF as a cell-type specific response to LPA and TGF-β in human tubular epithelial cells.Cell communication and signaling : CCS · 2012Article
- Laminin 511 partners with laminin 332 to mediate directional migration of Madin-Darby canine kidney epithelial cells.Molecular biology of the cell · 2012Article
- αVβ6 integrin promotes corneal wound healing.Investigative ophthalmology & visual science · 2011Article
- Integration of TGF-β- and EGFR-based signaling pathways using an agent-based model of epithelial restitution.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair SocietyArticle
- Transforming growth factor beta 1 activation, storage, and signaling pathways in idiopathic pulmonary fibrosis in dogs.Journal of veterinary internal medicineArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Laminin (LM)-332 is an extracellular matrix protein that plays a structural role in normal tissues and is also important in facilitating recovery of epithelia from injury. We have shown that expression of LM-332 is up-regulated during renal epithelial regeneration after ischemic injury, but the molecular signals that control expression are unknown. Here, we demonstrate that in Madin-Darby canine kidney (MDCK) epithelial cells LM-332 expression occurs only in subconfluent cultures and is turned-off after a polarized epithelium has formed. Addition of active transforming growth factor (TGF)-β1 to confluent MDCK monolayers is sufficient to induce transcription of the LM α3 gene and LM-332 protein expression via the TGF-β type I receptor (TβR-I) and the Smad2-Smad4 complex. Significantly, we show that expression of LM-332 in MDCK cells is an autocrine response to endogenous TGF-β1 secretion and activation mediated by integrin αVβ3 because neutralizing antibodies block LM-332 production in subconfluent cells. In confluent cells, latent TGF-β1 is secreted apically, whereas TβR-I and integrin αVβ3 are localized basolaterally. Disruption of the epithelial barrier by mechanical injury activates TGF-β1, leading to LM-332 expression. Together, our data suggest a novel mechanism for triggering the production of LM-332 after epithelial injury.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.