ArticleMolecular biology of the cell2010
Dynamics of multiple nuclei in Ashbya gossypii hyphae depend on the control of cytoplasmic microtubules length by Bik1, Kip2, Kip3, and not on a capture/shrinkage mechanism.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- Sporulation inJournal of fungi (Basel, Switzerland) · 2020Review
- Dynein is regulated by the stability of its microtubule track.The Journal of cell biology · 2017Article
- Mechanism of nuclear movements in a multinucleated cell.Molecular biology of the cell · 2017Article
- Nuclear autonomy in multinucleate fungi.Current opinion in microbiology · 2015Review
- Fungal model systems and the elucidation of pathogenicity determinants.Fungal genetics and biology : FG & B · 2014Review
- Role and regulation of kinesin-8 motors through the cell cycle.Systems and synthetic biology · 2014Article
- Organization of organelles within hyphae of Ashbya gossypii revealed by electron tomography.Eukaryotic cell · 2013Article
- Nuclear repulsion enables division autonomy in a single cytoplasm.Current biology : CB · 2013Article
- Genomes of Ashbya fungi isolated from insects reveal four mating-type loci, numerous translocations, lack of transposons, and distinct gene duplications.G3 (Bethesda, Md.) · 2013Article
- Move in for the kill: motile microtubule regulators.Trends in cell biology · 2012Review
- Clustering of nuclei in multinucleated hyphae is prevented by dynein-driven bidirectional nuclear movements and microtubule growth control in Ashbya gossypii.Eukaryotic cell · 2011Article
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2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Ashbya gossypii has a budding yeast-like genome but grows exclusively as multinucleated hyphae. In contrast to budding yeast where positioning of nuclei at the bud neck is a major function of cytoplasmic microtubules (cMTs), A. gossypii nuclei are constantly in motion and positioning is not an issue. To investigate the role of cMTs in nuclear oscillation and bypassing, we constructed mutants potentially affecting cMT lengths. Hyphae lacking the plus (+)end marker Bik1 or the kinesin Kip2 cannot polymerize long cMTs and lose wild-type nuclear movements. Interestingly, hyphae lacking the kinesin Kip3 display longer cMTs concomitant with increased nuclear oscillation and bypassing. Polymerization and depolymerization rates of cMTs are 3 times higher in A. gossypii than in budding yeast and cMT catastrophes are rare. Growing cMTs slide along the hyphal cortex and exert pulling forces on nuclei. Surprisingly, a capture/shrinkage mechanism seems to be absent in A. gossypii. cMTs reaching a hyphal tip do not shrink, and cMT +ends accumulate in hyphal tips. Thus, differences in cMT dynamics and length control between budding yeast and A. gossypii are key elements in the adaptation of the cMT cytoskeleton to much longer cells and much higher degrees of nuclear mobilities.
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