ArticleThe Journal of biological chemistry2010
The canonical BMP signaling pathway plays a crucial part in stimulation of dentin sialophosphoprotein expression by BMP-2.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 51 citations in OpenAlex.
- Human CD24Nature communications · 2026Article
- Crosstalk Between Hematopoietic and Mesenchymal Cells in Bone and Tooth Immune Response.Advances in experimental medicine and biology · 2026Review
- The Biofunctional Monomer, the Calcium Salt of 4-Methacryloxyethyl Trimellitic Acid, Promotes Odontoblast Differentiation in Three-Dimensional Culture System.International journal of biomaterials · 2025Article
- Effects of different signaling pathways on odontogenic differentiation of dental pulp stem cells: a review.Frontiers in physiology · 2023Review
- Review
- Article
- FGF8 and BMP2 mediated dynamic regulation of dental mesenchyme proliferation and differentiation via Lhx8/Suv39h1 complex.Journal of cellular and molecular medicine · 2021Article
- Efficacy of Photobiomodulation and Vitamin D on Odontogenic Activity of Human Dental Pulp Stem Cells.Journal of lasers in medical sciences · 2021Article
- Differential lncRNA/mRNA Expression Profiling and Functional Network Analyses in Bmp2 Deletion of Mouse Dental Papilla Cells.Frontiers in genetics · 2021Article
- Article
- LncRNA H19 promotes odontoblastic differentiation of human dental pulp stem cells by regulating miR-140-5p and BMP-2/FGF9.Stem cell research & therapy · 2020Article
- Identification of dentinogenic cell-specific surface antigens in odontoblast-like cells derived from adult dental pulp.Stem cell research & therapy · 2019Article
- Dental pulp stem cells and osteogenesis: an update.Cytotechnology · 2018Review
- Quaking promotes the odontoblastic differentiation of human dental pulp stem cells.Journal of cellular physiology · 2018Article
- Hey1 functions as a positive regulator of odontogenic differentiation in odontoblast‑lineage cells.International journal of molecular medicine · 2018Article
- BMP-2 induced Dspp transcription is mediated by Dlx3/Osx signaling pathway in odontoblasts.Scientific reports · 2017Article
- PfSMAD4 plays a role in biomineralization and can transduce bone morphogenetic protein-2 signals in the pearl oyster Pinctada fucata.BMC developmental biology · 2016Article
- Enhanced Dentinogenesis of Pulp Progenitors by Early Exposure to FGF2.Journal of dental research · 2015Article
- Establishment of Immortalized Mouse Bmp2 Knock-Out Dental Papilla Mesenchymal Cells Necessary for Study of Odontoblastic Differentiation and Odontogenesis.Journal of cellular physiology · 2015Article
- Transcriptional repression of the Dspp gene leads to dentinogenesis imperfecta phenotype in Col1a1-Trps1 transgenic mice.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2012Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dentin sialophosphoprotein (DSPP), a typical dentin-specific protein, is mainly expressed in the dentin extracellular matrix and plays a role in dentin mineralization. BMP-2 provides a strong signal for differentiation and mineralization of odontoblasts and osteoblasts. Previously, BMP-2 treatment is reported to stimulate Dspp expression in the MD10-F2 pre-odontoblast cells through activation of the heterotrimeric transcription factor Y (NF-Y). The canonical BMP signaling pathway is known to contribute greatly to biomineralization, however, it is not known whether it is involved in Dspp expression. Here, we investigated this question. Activation of the canonical BMP-2 signaling pathway in MDPC-23, preodontoblast cell, by overexpression of constitutively active Smad1/5 or downstream transcription factors Dlx5 and Runx2 stimulated Dspp expression. Conversely, knockdown of each element with siRNA significantly blocked the BMP-2-induced Dspp expression. To test whether these transcription factors downstream of BMP-2 are directly involved in regulating Dspp, we analyzed the mouse Dspp promoter. There are 5 well conserved homeodomain binding elements, H1 to H5, in Dspp proximal promoter regions (-791 to +54). A serial deletion of H1 and H2 greatly changed basal promoter activity and responsiveness to Dlx5 or Msx2. However, further deletions did not change the responsiveness to Dlx5 or Msx2. H1 and H2 sites can be suggested as specific response elements of Dlx5 and Msx2, respectively, based on their promoter activity modulation. Thus, the canonical BMP-2 signaling pathway plays a crucial part in the regulation of Dspp expression through the action of Smads, Dlx5, Runx2, and Msx2.
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