ArticleMolecular biology reports2011
Molecular and biochemical analysis of the α class carbonic anhydrases in Caenorhabditis elegans.
Article in Molecular biology reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 41 citations in OpenAlex.
- The carbonic anhydrase of Clostridium autoethanogenum represents a new subclass of β-carbonic anhydrases.Applied microbiology and biotechnology · 2019Article
- A Novel Mechanism of pH Buffering in C. elegans Glia: Bicarbonate Transport via the Voltage-Gated ClC Cl- Channel CLH-1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- β carbonic anhydrase is required for female fertility in Drosophila melanogaster.Frontiers in zoology · 2015Article
- Insights into bacterial CO2 metabolism revealed by the characterization of four carbonic anhydrases in Ralstonia eutropha H16.AMB Express · 2014Article
- Carnosine: can understanding its actions on energy metabolism and protein homeostasis inform its therapeutic potential?Chemistry Central journal · 2013Article
- CAS-1, a C. elegans cyclase-associated protein, is required for sarcomeric actin assembly in striated muscle.Journal of cell science · 2012Article
- Identification of a nuclear carbonic anhydrase in Caenorhabditis elegans.Biochimica et biophysica acta · 2012Article
- Sulfurous gases as biological messengers and toxins: comparative genetics of their metabolism in model organisms.Journal of toxicology · 2011Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, in silico analysis of the Caenorhabditis elegans genome revealed six genes (cah-1, cah-2, cah-3, cah-4, cah-5, and cah-6) possibly encoding α class CAs (carbonic anhydrase). Real-time RT-PCR analysis revealed the temporal expression pattern of each gene, as well as changes in expression levels under different atmospheric conditions (stress). Cah-3 and cah-4 showed the highest levels of transcript accumulation, while most genes responded to the stress conditions. Yeast complementation showed that cah-3 was able to complement the function of Saccharomyces cerevisiae CA (NCE103) in vivo. Recombinant CAH-3, CAH-4a and CAH-5 enzymes, expressed in Escherichia coli were used for in vitro measurement of CA activity. However, in vitro activity was only detectable for CAH-4a. RNAi by feeding was performed on wild-type C. elegans for all genes. The worms were examined for a visible phenotype under normal and stress conditions (pH, CO(2)/O(2)). Silencing cah-3 and cah-4 may reduce the life-span of the worms (at 22 °C).
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