ArticleJournal of molecular endocrinology2010
Involvement of the orphan nuclear estrogen receptor-related receptor α in osteoclast adhesion and transmigration.
Article in Journal of molecular endocrinology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- ERRα: unraveling its role as a key player in cell migration.Oncogene · 2024Review
- Effects of β2 Integrins on Osteoclasts, Macrophages, Chondrocytes, and Synovial Fibroblasts in Osteoarthritis.Biomolecules · 2022Review
- Estrogen-Related Receptor α: A Significant Regulator and Promising Target in Bone Homeostasis and Bone Metastasis.Molecules (Basel, Switzerland) · 2022Review
- PGC-1: a key regulator in bone homeostasis.Journal of bone and mineral metabolism · 2022Review
- Effects of Estrogens on Osteoimmunology: A Role in Bone Metastasis.Frontiers in immunology · 2022Review
- Estrogen-related receptors: novel potential regulators of osteoarthritis pathogenesis.Molecular medicine (Cambridge, Mass.) · 2021Review
- The Macrophage-Osteoclast Axis in Osteoimmunity and Osteo-Related Diseases.Frontiers in immunology · 2021Review
- Molecular determinants for the polarization of macrophage and osteoclast.Seminars in immunopathology · 2019Review
- ERRα promotes breast cancer cell dissemination to bone by increasing RANK expression in primary breast tumors.Oncogene · 2019Article
- Regulation of osteogenesis and osteoclastogenesis by zoledronic acid loaded on biodegradable magnesium-strontium alloy.Scientific reports · 2019Article
- ERRα negatively regulates type I interferon induction by inhibiting TBK1-IRF3 interaction.PLoS pathogens · 2017Article
- The estrogen-related receptors (ERRs): potential targets against bone loss.Cellular and molecular life sciences : CMLS · 2016Review
- Minireview: nuclear receptor regulation of osteoclast and bone remodeling.Molecular endocrinology (Baltimore, Md.) · 2015Review
- Tools of the trade: podosomes as multipurpose organelles of monocytic cells.Cellular and molecular life sciences : CMLS · 2015Review
- Cartilage-specific overexpression of ERRγ results in Chondrodysplasia and reduced chondrocyte proliferation.PloS one · 2013Article
- RNA therapeutics targeting osteoclast-mediated excessive bone resorption.Advanced drug delivery reviews · 2012Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The orphan nuclear receptor, estrogen receptor-related receptor α (ERRα) is expressed in osteoblasts and osteoclasts (OCs) and has been proposed to be a modulator of estrogen signaling. To determine the role of ERRα in OC biology, we knocked down ERRα activity by transient transfection of an siRNA directed against ERRα in the RAW264.7 monocyte-macrophage cell line that differentiates into OCs in the presence of receptor activator of nuclear factor κB-ligands and macrophage colony-stimulating factor. In parallel, stable RAW cell lines expressing a dominant-negative form of ERRα and green fluorescent protein (RAW-GFP-ERRαΔAF2) were used. Expression of OC markers was assessed by real-time PCR, and adhesion and transmigration tests were performed. Actin cytoskeletal organization was visualized using confocal microscopy. We found that RAW264.7 cells expressing siRNA directed against ERRα and RAW-GFP-ERRαΔAF2 OCs displayed abnormal spreading, and decreased osteopontin and β3 integrin subunit expression compared with the corresponding control cells. Decreased adhesion and the absence of podosome belts concomitant with abnormal localization of c-src were also observed in RAW-GFP-ERRαΔAF2-derived OCs. In addition, RAW-GFP-ERRαΔAF2-derived OCs failed to transmigrate through osteoblast cell layers. Our data show that the impairment of ERRα function does not alter OC precursor proliferation and differentiation but does alter the adhesion/spreading and migration capacities of mature OCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.