ArticleMolecular cytogenetics2010
Fluorescence in situ hybridization in combination with the comet assay and micronucleus test in genetic toxicology.
Article in Molecular cytogenetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 71 citations in OpenAlex.
- Cytological changes in the oral mucosa after use of a mouth rinse with alcohol. A prospective double blind control study.Medicina oral, patologia oral y cirugia bucal · 2012Trial
- Radon-Induced Radiation Biomarkers: A Scoping Review from Exposure Dosimetry to Early Biological Effects on the Lung.International journal of molecular sciences · 2026Article
- Genotoxicity Comparison betweenFoods (Basel, Switzerland) · 2022Article
- Determination of Genotoxicity Attributed to Diesel Exhaust Particles in Normal Human Embryonic Lung Cell (WI-38) Line.Biomolecules · 2021Article
- Molecular Cytogenetics in the Era of Chromosomics and Cytogenomic Approaches.Frontiers in genetics · 2021Review
- Analysis of Xq27.3 Fragility Using the Micronucleus-FluorescenceGenome integrity · 2020Article
- Distinctive fingerprints of genotoxicity induced by As, Cr, Cd, and Ni in a freshwater fish.Environmental science and pollution research international · 2019Article
- Article
- Genomic Organization Under Different Environmental Conditions: Hoplosternum Littorale as a Model.Zebrafish · 2016Article
- The use of comet assay in plant toxicology: recent advances.Frontiers in genetics · 2015Review
- New developments in comet-FISH.Mutagenesis · 2015Article
- Toxoplasma gondii infection can induce retinal DNA damage: an experimental study.International journal of ophthalmology · 2014Article
- Recent advances in in vivo genotoxicity testing: prediction of carcinogenic potential using comet and micronucleus assay in animal models.Journal of cancer prevention · 2013Review
- Molecular Cytogenetics: the first impact factor (2.36).Molecular cytogenetics · 2013Article
- Assaying DNA damage in hippocampal neurons using the comet assay.Journal of visualized experiments : JoVE · 2012Article
- Evaluation of DNA single and double strand breaks in women with cervical neoplasia based on alkaline and neutral comet assay techniques.Journal of biomedicine & biotechnology · 2012Article
- New application of the comet assay: chromosome--comet assay.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2011Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Comet assay and micronucleus (MN) test are widely applied in genotoxicity testing and biomonitoring. While comet assay permits to measure direct DNA-strand breaking capacity of a tested agent MN test allows estimating the induced amount of chromosome and/or genome mutations. The potential of these two methods can be enhanced by the combination with fluorescence in situ hybridization (FISH) techniques. FISH plus comet assay allows the recognition of targets of DNA damage and repairing directly. FISH combined with MN test is able to characterize the occurrence of different chromosomes in MN and to identify potential chromosomal targets of mutagenic substances. Thus, combination of FISH with the comet assay or MN test proved to be promising techniques for evaluation of the distribution of DNA and chromosome damage in the entire genome of individual cells. FISH technique also permits to study comet and MN formation, necessary for correct application of these methods. This paper reviews the relevant literature on advantages and limitations of Comet-FISH and MN-FISH assays application in genetic toxicology.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.