ArticleInternational wound journal2010
Keloid explant culture: a model for keloid fibroblasts isolation and cultivation based on the biological differences of its specific regions.
Article in International wound journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- A serum-free adipose-conditioned medium delays stem cell senescence and maintains tissue homeostasis via IL-6/STAT3 axis suppression.Stem cell research & therapy · 2025Article
- Therapeutic Potential of Adipose-Derived Stem Cell-Conditioned Medium and Extracellular Vesicles in an In Vitro Radiation-Induced Skin Injury Model.International journal of molecular sciences · 2023Article
- Therapeutic Potential of Adipose Stem Cell-Derived Conditioned Medium on Scar Contraction Model.Biomedicines · 2022Article
- [Research advances on the characteristics of fibroblast in keloid].Zhonghua shao shang yu chuang mian xiu fu za zhi · 2022Article
- Article
- Construction of a HOXA11-AS-Interact Ed Network in Keloid Fibroblasts Using Integrated Bioinformatic Analysis andFrontiers in genetics · 2022Article
- Long-term explant culture: an improved method for consistently harvesting homogeneous populations of keloid fibroblasts.Bioengineered · 2022Article
- Secreted Factors from Keloid Keratinocytes Modulate Collagen Deposition by Fibroblasts from Normal and Fibrotic Tissue: A Pilot Study.Biomedicines · 2020Article
- Identification of Differentially Methylated CpG Sites in Fibroblasts from Keloid Scars.Biomedicines · 2020Article
- The Keloid Disorder: Heterogeneity, Histopathology, Mechanisms and Models.Frontiers in cell and developmental biology · 2020Review
- Review
- Role of Hypoxia Inducible Factor-1 Alpha (HIF-1α) in Cytoglobin Expression and Fibroblast Proliferation of Keloids.The Kobe journal of medical sciences · 2019Article
- Reconstructed human keloid models show heterogeneity within keloid scars.Archives of dermatological research · 2018Article
- Sorafenib exerts an anti-keloid activity by antagonizing TGF-β/Smad and MAPK/ERK signaling pathways.Journal of molecular medicine (Berlin, Germany) · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro studies with keloid fibroblasts frequently present contradictory results. This may occur because keloids present distinct genotypic and phenotypic characteristics in its different regions, such as the peripheral region in relation to the central region. We suggest an explant model for keloid fibroblasts harvesting, standardising the initial processing of keloid samples to obtain fragments from different regions, considering its biological differences, for primary cell culture. The different keloid regions were delimited and fragments were obtained using a 3-mm diameter punch. To remove fragments from the periphery, the punch was placed in one longitudinal line extremity, respecting the lesion borders. For the central region, it was placed in the intersection of lines at the level of the largest longitudinal and transversal axes, the other fragments being removed centrifugally in relation to the first one. Primary fibroblast culture was carried out by explant. Flow cytometry analysis showed cell cycle differences between the groups, confirming its different origins and biological characteristics. In conclusion, our proposed model proved itself efficient for keloid fibroblast isolation from specific regions and cultivation. Its simplicity and ease of execution may turn it into an important tool for studying the characteristics of the different keloid-derived fibroblasts in culture.
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