ArticleJournal of orthopaedic research : official publication of the Orthopaedic Research Society2010
Prostaglandin E2 and prostaglandin F2α differentially modulate matrix metabolism of human nucleus pulposus cells.
Article in Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
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- Extracellular matrix in intervertebral disc: basic and translational implications.Cell and tissue research · 2022Review
- Investigation into the anti-inflammatory properties of metformin in intervertebral disc cells.JOR spine · 2022Article
- Actions of Prostaglandins on Human Nucleus Pulposus Metabolism Inferred by Cyclooxygenase 2 Inhibition of Cytokine Activated Cells.Neurospine · 2020Article
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- NSAID use in intervertebral disc degeneration: what are the effects on matrix homeostasis in vivo?The spine journal : official journal of the North American Spine Society · 2017Article
- Inflammatory Processes Associated with Canine Intervertebral Disc Herniation.Frontiers in immunology · 2017Article
- Inflammatory profiles in canine intervertebral disc degeneration.BMC veterinary research · 2016Article
- Biocompatibility and intradiscal application of a thermoreversible celecoxib-loaded poly-N-isopropylacrylamide MgFe-layered double hydroxide hydrogel in a canine model.Arthritis research & therapy · 2015Article
- Connexin43 hemichannels mediate small molecule exchange between chondrocytes and matrix in biomechanically-stimulated temporomandibular joint cartilage.Osteoarthritis and cartilage · 2014Article
- The effect of a cyclooxygenase 2 inhibitor on early degenerated human nucleus pulposus explants.Global spine journal · 2014Article
- Spine degeneration in a murine model of chronic human tobacco smokers.Osteoarthritis and cartilage · 2012Article
- Micro Fragmented Adipose Tissue Promotes the Matrix Synthesis Function of Nucleus Pulposus Cells and Regenerates Degenerated Intervertebral Disc in a Pig Model.Cell transplantationArticle
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
Prostaglandin (PG) actions on disc metabolism are unclear even though certain PGs are highly expressed by disc cells under inflammatory conditions and nonsteroidal anti-inflammatory drugs (NSAIDs) are frequently used to block PG production to treat back pain. Hence this study aimed to (1) quantify gene expression of arachidonic acid cascade components responsible for PG synthesis and (2) examine the effects of key PGs on disc matrix homeostasis. Microarray analysis revealed that inflammatory stress increases expression of synthases and receptors for prostaglandin E2 (PGE(2)) and prostaglandin F2α (PGF(2α)), resulting in elevated PGE(2) and PGF(2α) production in conditioned media of disc cells. PGE(2) diminished disc cell proteoglycan synthesis, in a dose-dependent manner. Semiquantitative RT-PCR revealed differential effects of PGE(2) and PGF(2α) on disc cell expression of key matrix structural genes, aggrecan, versican, collagens type I and II. PGE(2) and PGF(2α) also decreased message for the anabolic factor, IGF-1. PGE(2) decreased mRNA expression for the anti-catabolic factor TIMP-1 while PGF(2α) increased mRNAs for catabolic factors MMP-1 and MMP-3. Thus, PGE(2) and PGF(2α) may have an overall negative impact on disc matrix homeostasis, and the use of NSAIDs may impact disc metabolism as well as treat back pain.
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Registered trials
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