ArticlePLoS genetics2010
The baker's yeast diploid genome is remarkably stable in vegetative growth and meiosis.
Article in PLoS genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed.
- The impact of evolutionary dynamics and expression plasmid stability on long-term heterologous protein production by yeast.Microbial cell factories · 2026Article
- Roles of Mutation, Ploidy, and Recombination in Adaptive Evolution in Two Divergent Model Yeasts.Genes · 2026Review
- Footprints of Human Migration in the Population Structure of Wild Baker's Yeast.Molecular ecology · 2025Article
- The dynamics of loss of heterozygosity events in genomes.EMBO reports · 2025Review
- Patterns of spontaneous and induced genomic alterations inApplied and environmental microbiology · 2025Article
- Wasp intestinal cues drive yeast toward outbreeding strategies.The ISME journal · 2025Article
- Instability throughout the Saccharomyces cerevisiae genome resulting from Pms1 endonuclease deficiency.Nucleic acids research · 2024Article
- A widespread inversion polymorphism conserved among Saccharomyces species is caused by recurrent homogenization of a sporulation gene family.PLoS genetics · 2022Article
- Overdominant and partially dominant mutations drive clonal adaptation in diploid Saccharomyces cerevisiae.Genetics · 2022Article
- ESA1 regulates meiotic chromosome axis and crossover frequency via acetylating histone H4.Nucleic acids research · 2021Article
- Loss of heterozygosity results in rapid but variable genome homogenization across yeast genetic backgrounds.eLife · 2021Article
- The fidelity of DNA replication, particularly on GC-rich templates, is reduced by defects of the Fe-S cluster in DNA polymerase δ.Nucleic acids research · 2021Article
- Stability across the Whole Nuclear Genome in the Presence and Absence of DNA Mismatch Repair.Cells · 2021Review
- Review
- Mutagenic mechanisms of cancer-associated DNA polymerase ϵ alleles.Nucleic acids research · 2021Article
- Hundreds of thousands of cell generations reveal a treasure chest of genome alterations.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Genome-wide mapping of spontaneous genetic alterations in diploid yeast cells.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Variable Spontaneous Mutation and Loss of Heterozygosity among Heterozygous Genomes in Yeast.Molecular biology and evolution · 2020Article
- Trajectory and uniqueness of mutational signatures in yeast mutators.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Loss of Heterozygosity and Base Mutation Rates Vary AmongG3 (Bethesda, Md.) · 2020Article
10 more citing papers are in PubMed but not listed here.
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12 authors.
Funding
Abstract
Accurate estimates of mutation rates provide critical information to analyze genome evolution and organism fitness. We used whole-genome DNA sequencing, pulse-field gel electrophoresis, and comparative genome hybridization to determine mutation rates in diploid vegetative and meiotic mutation accumulation lines of Saccharomyces cerevisiae. The vegetative lines underwent only mitotic divisions while the meiotic lines underwent a meiotic cycle every ∼20 vegetative divisions. Similar base substitution rates were estimated for both lines. Given our experimental design, these measures indicated that the meiotic mutation rate is within the range of being equal to zero to being 55-fold higher than the vegetative rate. Mutations detected in vegetative lines were all heterozygous while those in meiotic lines were homozygous. A quantitative analysis of intra-tetrad mating events in the meiotic lines showed that inter-spore mating is primarily responsible for rapidly fixing mutations to homozygosity as well as for removing mutations. We did not observe 1-2 nt insertion/deletion (in-del) mutations in any of the sequenced lines and only one structural variant in a non-telomeric location was found. However, a large number of structural variations in subtelomeric sequences were seen in both vegetative and meiotic lines that did not affect viability. Our results indicate that the diploid yeast nuclear genome is remarkably stable during the vegetative and meiotic cell cycles and support the hypothesis that peripheral regions of chromosomes are more dynamic than gene-rich central sections where structural rearrangements could be deleterious. This work also provides an improved estimate for the mutational load carried by diploid organisms.
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