ArticlePLoS computational biology2010
Minimal mesoscale model for protein-mediated vesiculation in clathrin-dependent endocytosis.
Article in PLoS computational biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 55 citations in OpenAlex.
- Dynamics of the formation of flat clathrin lattices in response to growth factor stimulus.PLoS computational biology · 2026Article
- Dynamics of the formation of flat clathrin lattices in response to growth factor stimulus.bioRxiv : the preprint server for biology · 2025Article
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- Asymmetric crowders and membrane morphology at the nexus of intracellular trafficking and oncology.Mechanobiology in medicine · 2024Article
- Free energy calculations for membrane morphological transformations and insights to physical biology and oncology.Methods in enzymology · 2024Article
- Mem3DG: Modeling membrane mechanochemical dynamics in 3D using discrete differential geometry.Biophysical reports · 2022Article
- Data driven and biophysical insights into the regulation of trafficking vesicles by extracellular matrix stiffness.iScience · 2022Article
- Quantification of Curvature Sensing Behavior of Curvature-Inducing Proteins on Model Wavy Substrates.The Journal of membrane biology · 2022Article
- Large self-assembled clathrin lattices spontaneously disassemble without sufficient adaptor proteins.PLoS computational biology · 2022Article
- Dynamic interplay between cell membrane tension and clathrin-mediated endocytosis.Biology of the cell · 2021Review
- Nonaxisymmetric Shapes of Biological Membranes from Locally Induced Curvature.Biophysical journal · 2020Article
- Article
- Emergent membrane morphologies in relaxed and tense membranes in presence of reversible adhesive pinning interactions.Physical biology · 2019Article
- Biophysics of membrane curvature remodeling at molecular and mesoscopic lengthscales.Journal of physics. Condensed matter : an Institute of Physics journal · 2018Article
- Clathrin polymerization exhibits high mechano-geometric sensitivity.Soft matter · 2017Article
- Curvature-undulation coupling as a basis for curvature sensing and generation in bilayer membranes.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Cargo binding promotes KDEL receptor clustering at the mammalian cell surface.Scientific reports · 2016Article
- Thermodynamic free energy methods to investigate shape transitions in bilayer membranes.International journal of advances in engineering sciences and applied mathematics · 2016Article
- Membrane Mechanics of Endocytosis in Cells with Turgor.PLoS computational biology · 2015Article
- Endocytic proteins drive vesicle growth via instability in high membrane tension environment.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In eukaryotic cells, the internalization of extracellular cargo via the endocytic machinery is an important regulatory process required for many essential cellular functions. The role of cooperative protein-protein and protein-membrane interactions in the ubiquitous endocytic pathway in mammalian cells, namely the clathrin-dependent endocytosis, remains unresolved. We employ the Helfrich membrane Hamiltonian together with surface evolution methodology to address how the shapes and energetics of vesicular-bud formation in a planar membrane are stabilized by presence of the clathrin-coat assembly. Our results identify a unique dual role for the tubulating protein epsin: multiple epsins localized spatially and orientationally collectively play the role of a curvature inducing capsid; in addition, epsin serves the role of an adapter in binding the clathrin coat to the membrane. Our results also suggest an important role for the clathrin lattice, namely in the spatial- and orientational-templating of epsins. We suggest that there exists a critical size of the coat above which a vesicular bud with a constricted neck resembling a mature vesicle is stabilized. Based on the observed strong dependence of the vesicle diameter on the bending rigidity, we suggest that the variability in bending stiffness due to variations in membrane composition with cell type can explain the experimentally observed variability on the size of clathrin-coated vesicles, which typically range 50-100 nm. Our model also provides estimates for the number of epsins involved in stabilizing a coated vesicle, and without any direct fitting reproduces the experimentally observed shapes of vesicular intermediates as well as their probability distributions quantitatively, in wildtype as well as CLAP IgG injected neuronal cell experiments. We have presented a minimal mesoscale model which quantitatively explains several experimental observations on the process of vesicle nucleation induced by the clathrin-coated assembly prior to vesicle scission in clathrin dependent endocytosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.