ArticleGenetics2010
Telomerase recruitment in Saccharomyces cerevisiae is not dependent on Tel1-mediated phosphorylation of Cdc13.
Article in Genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 52 citations in OpenAlex.
- The canonical RPA complex interacts with Est3 to regulate yeast telomerase activity.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Suppression of telomere capping defects of Saccharomyces cerevisiae yku70 and yku80 mutants by telomerase.G3 (Bethesda, Md.) · 2021Article
- The role of Rif1 in telomere length regulation is separable from its role in origin firing.eLife · 2020Article
- Tel1/ATM Signaling to the Checkpoint Contributes to Replicative Senescence in the Absence of Telomerase.Genetics · 2019Article
- Quantitative assessment of changes in cell growth, size and morphology during telomere-initiated cellular senescence in Saccharomyces cerevisiae.Experimental cell research · 2019Article
- Fine tuning the level of the Cdc13 telomere-capping protein for maximal chromosome stability performance.Current genetics · 2019Review
- Rif1 acts through Protein Phosphatase 1 but independent of replication timing to suppress telomere extension in budding yeast.Nucleic acids research · 2018Article
- Article
- A sharp Pif1-dependent threshold separates DNA double-strand breaks from critically short telomeres.eLife · 2017Article
- Regulation of telomere metabolism by the RNA processing protein Xrn1.Nucleic acids research · 2017Article
- Rad6-Bre1-mediated H2B ubiquitination regulates telomere replication by promoting telomere-end resection.Nucleic acids research · 2017Article
- Regulating telomere length from the inside out: the replication fork model.Genes & development · 2016Article
- Rif1: A Conserved Regulator of DNA Replication and Repair Hijacked by Telomeres in Yeasts.Frontiers in genetics · 2016Article
- Requirement of the FATC domain of protein kinase Tel1 for localization to DNA ends and target protein recognition.Molecular biology of the cell · 2015Article
- At short telomeres Tel1 directs early replication and phosphorylates Rif1.PLoS genetics · 2014Article
- Multifunctional role of ATM/Tel1 kinase in genome stability: from the DNA damage response to telomere maintenance.BioMed research international · 2014Review
- Telomere-end processing: mechanisms and regulation.Chromosoma · 2013Article
- Multiple genetic pathways regulate replicative senescence in telomerase-deficient yeast.Aging cell · 2013Article
- Tel1 and Rad51 are involved in the maintenance of telomeres with capping deficiency.Nucleic acids research · 2013Article
- A hypomorphic mutation reveals a stringent requirement for the ATM checkpoint protein in telomere protection during early cell division in Drosophila.G3 (Bethesda, Md.) · 2013Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
In Saccharomyces cerevisiae, association between the Est1 telomerase subunit and the telomere-binding protein Cdc13 is essential for telomerase to be recruited to its site of action. A current model proposes that Tel1 binding to telomeres marks them for elongation, as the result of phosphorylation of a proposed S/TQ cluster in the telomerase recruitment domain of Cdc13. However, three observations presented here argue against one key aspect of this model. First, the pattern of Cdc13 phosphatase-sensitive isoforms is not altered by loss of Tel1 function or by mutations introduced into two conserved serines (S249 and S255) in the Cdc13 recruitment domain. Second, an interaction between Cdc13 and Est1, as monitored by a two-hybrid assay, is dependent on S255 but Tel1-independent. Finally, a derivative of Cdc13, cdc13-(S/TQ)11→(S/TA)11, in which every potential consensus phosphorylation site for Tel1 has been eliminated, confers nearly wild-type telomere length. These results are inconsistent with a model in which the Cdc13-Est1 interaction is regulated by Tel1-mediated phosphorylation of the Cdc13 telomerase recruitment domain. We propose an alternative model for the role of Tel1 in telomere homeostasis, which is based on the assumption that Tel1 performs the same molecular task at double-strand breaks (DSBs) and chromosome termini.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.