ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2011
Myosin cleft closure determines the energetics of the actomyosin interaction.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- The hypertrophic cardiomyopathy mutation G768R makes cardiac myosin a high duty ratio motor.bioRxiv : the preprint server for biology · 2026Article
- Homologous mutations in human β, embryonic, and perinatal muscle myosins have divergent effects on molecular power generation.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Actomyosin Complex.Sub-cellular biochemistry · 2022Article
- Parallel Compression Is a Fast Low-Cost Assay for the High-Throughput Screening of Mechanosensory Cytoskeletal Proteins in Cells.ACS applied materials & interfaces · 2017Article
- Molecular mechanisms underlying deoxy-ADP.Pi activation of pre-powerstroke myosin.Protein science : a publication of the Protein Society · 2017Article
- Statistical Thermodynamics for Actin-Myosin Binding: The Crucial Importance of Hydration Effects.Biophysical journal · 2016Article
- Cell shape regulation through mechanosensory feedback control.Journal of the Royal Society, Interface · 2015Article
- Structural model of weak binding actomyosin in the prepowerstroke state.The Journal of biological chemistry · 2015Article
- Poorly understood aspects of striated muscle contraction.BioMed research international · 2015Review
- Sucrose increases the activation energy barrier for actin-myosin strong binding.Archives of biochemistry and biophysics · 2014Article
- A subdomain interaction at the base of the lever allosterically tunes the mechanochemical mechanism of myosin 5a.PloS one · 2013Article
- Myosin and tropomyosin stabilize the conformation of formin-nucleated actin filaments.The Journal of biological chemistry · 2012Article
- A novel actin binding site of myosin required for effective muscle contraction.Nature structural & molecular biology · 2012Article
- Understanding the cooperative interaction between myosin II and actin cross-linkers mediated by actin filaments during mechanosensation.Biophysical journal · 2012Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Formation of the strong binding interaction between actin and myosin is essential for force generation in muscle and in cytoskeletal motor systems. To clarify the role of the closure of myosin's actin-binding cleft in the actomyosin interaction, we performed rapid kinetic, spectroscopic, and calorimetric experiments and atomic-level energetic calculations on a variety of myosin isoforms for which atomic structures are available. Surprisingly, we found that the endothermic actin-binding profile of vertebrate skeletal muscle myosin subfragment-1 is unique among studied myosins. We show that the diverse propensity of myosins for cleft closure determines different energetic profiles as well as structural and kinetic pathways of actin binding. Depending on the type of myosin, strong actin binding may occur via induced-fit or conformational preselection mechanisms. However, cleft closure does not directly determine the kinetics and affinity of actin binding. We also show that cleft closure is enthalpically unfavorable, reflecting the development of an internal strain within myosin in order to adopt precise steric complementarity to the actin filament. We propose that cleft closure leads to an increase in the torsional strain of myosin's central β-sheet that has been proposed to serve as an allosteric energy-transducing spring during force generation.
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