ArticleProceedings of the National Academy of Sciences of the United States of America2010
Triton X-100 concentration effects on membrane permeability of a single HeLa cell by scanning electrochemical microscopy (SECM).
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 123 papers.
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Who cites it
123 citing papers in PubMed, 402 citations in OpenAlex.
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- Efficient high-quality and high molecular weight plant DNA extraction protocol using Percoll™.Plant biotechnology (Tokyo, Japan) · 2026Article
- Enhanced Solubilization of Ketoprofen Using Triton X-100, Triton X-165, Brij C10, and Their Binary and Ternary Mixed Micelle Systems.Pharmaceutical research · 2026Article
- Detergent-Triggered Membrane Remodelling Monitored via Intramembrane Fluorescence Dequenching.ACS omega · 2026Article
- Optimized Viability-ddPCR with Triton X-100 Enhancement for Selective Detection of LiveJournal of microbiology and biotechnology · 2026Article
- Alteration of CD40 Protein Expression by Tyrosine Phosphorylation of the NF-κB p50 Subunit.Chembiochem : a European journal of chemical biology · 2026Article
- C14DM Ablation Leads to Reduced Tolerance to Plasma Membrane Stress and Increased Drug Sensitivity inInternational journal of molecular sciences · 2025Article
- Mechanotransduction Activates Microglia and Impairs Phagocytosis in Stiff Amyloid-β Plaques.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Growth and gene expression of Pseudomonas nitroreducens TX1 on octylphenol polyethoxylate surfactants.Applied microbiology and biotechnology · 2025Article
- Intracellular delivery of proteins for live cell imaging.Journal of controlled release : official journal of the Controlled Release Society · 2025Article
- Saponin Improves Recovery of Bacteria from Orthopaedic Implants for Enhanced Diagnosis Ex Vivo.Microorganisms · 2025Article
- Advances and Challenges of Tissue Vascular Scaffolds and Supercritical Carbon Dioxide Technology in Cardiovascular Diseases.Tissue engineering and regenerative medicine · 2025Review
- Article
- Optimization and Validation of Maceration-Mediated Hydrodistillation to Extract Caryophyllene-Rich Essential Oil from Sea Buckthorn Berries.Food technology and biotechnology · 2025Article
- Application of decellularization methods for scaffold production: advantages, disadvantages, biosafety and modifications.Frontiers in bioengineering and biotechnology · 2025Review
- Genomic and functional insights intoFrontiers in microbiology · 2025Article
- The activity of cell-free supernatant ofFrontiers in cellular and infection microbiology · 2025Article
- Evaluation of effectiveness of bacteriophage purification methods.Virology journal · 2024Article
- Triton X-100-treated virus-based ELLA demonstrates discordant antigenic evolution of influenza B virus hemagglutinin and neuraminidase.Journal of virology · 2024Article
63 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Changes in HeLa cell morphology, membrane permeability, and viability caused by the presence of Triton X-100 (TX100), a nonionic surfactant, were studied by scanning electrochemical microscopy (SECM). No change in membrane permeability was found at concentrations of 0.15 mM or lower during an experimental period of 30 to 60 min. Permeability of the cell membrane to the otherwise impermeable, highly charged hydrophilic molecule ferrocyanide was seen starting at concentrations of TX100 of about 0.17 mM. This concentration level of TX100 did not affect cell viability. Based on a simulation model, the membrane permeability for ferrocyanide molecules passing though the live cell membrane was 6.5 ± 2.0 × 10(-6) m/s. Cells underwent irreversible permeabilization of the membrane and structural collapse when the TX100 concentration reached the critical micelle concentration (CMC), in the range of 0.19 to 0.20 mM. The impermeability of ferrocyanide molecules in the absence of surfactant was also used to determine the height and diameter of a single living cell with the aid of the approach curve and probe scan methods in SECM.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.