ArticleProceedings of the National Academy of Sciences of the United States of America2010
Deletion of the Cel48S cellulase from Clostridium thermocellum.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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42 citing papers in PubMed, 114 citations in OpenAlex.
- Mechanochemical Coupling of Catalysis and Motion in a Cellulose-Degrading Multienzyme Nanomachine.ACS catalysis · 2024Article
- Processive Enzymes Kept on a Leash: How Cellulase Activity in Multienzyme Complexes Directs Nanoscale Deconstruction of Cellulose.ACS catalysis · 2021Article
- Enhanced Activity by Genetic Complementarity: Heterologous Secretion of Clostridial Cellulases byMolecules (Basel, Switzerland) · 2021Article
- A Biological Nanomachine at Work: Watching the Cellulosome Degrade Crystalline Cellulose.ACS central science · 2020Article
- CRISPR interference (CRISPRi) as transcriptional repression tool for Hungateiclostridium thermocellum DSM 1313.Microbial biotechnology · 2020Article
- Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization.Biotechnology for biofuels · 2019Article
- The GH10 and GH48 dual-functional catalytic domains from a multimodular glycoside hydrolase synergize in hydrolyzing both cellulose and xylan.Biotechnology for biofuels · 2019Article
- Determination of the native features of the exoglucanase Cel48S fromBiotechnology for biofuels · 2018Article
- Isotope-Assisted Metabolite Analysis Sheds Light on Central Carbon Metabolism of a Model Cellulolytic BacteriumFrontiers in microbiology · 2018Article
- Efficient whole-cell-catalyzing cellulose saccharification using engineeredBiotechnology for biofuels · 2017Article
- Development of a plasmid-based expression system inMetabolic engineering communications · 2016Article
- Identifying promoters for gene expression inMetabolic engineering communications · 2015Article
- C/N ratio drives soil actinobacterial cellobiohydrolase gene diversity.Applied and environmental microbiology · 2015Article
- The bifunctional alcohol and aldehyde dehydrogenase gene, adhE, is necessary for ethanol production in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum.Journal of bacteriology · 2015Article
- Metatranscriptomic analyses of plant cell wall polysaccharide degradation by microorganisms in the cow rumen.Applied and environmental microbiology · 2015Article
- Genomic analysis of Chthonomonas calidirosea, the first sequenced isolate of the phylum Armatimonadetes.The ISME journal · 2014Article
- Characterization and evolution of tetrameric photosystem I from the thermophilic cyanobacterium Chroococcidiopsis sp TS-821.The Plant cell · 2014Article
- Deletion of Caldicellulosiruptor bescii CelA reveals its crucial role in the deconstruction of lignocellulosic biomass.Biotechnology for biofuels · 2014Article
- Comparison of transcriptional profiles of Clostridium thermocellum grown on cellobiose and pretreated yellow poplar using RNA-Seq.Frontiers in microbiology · 2014Article
- The contribution of cellulosomal scaffoldins to cellulose hydrolysis by Clostridium thermocellum analyzed by using thermotargetrons.Biotechnology for biofuels · 2014Article
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10 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Clostridium thermocellum is a thermophilic anaerobic bacterium that rapidly solubilizes cellulose with the aid of a multienzyme cellulosome complex. Creation of knockout mutants for Cel48S (also known as CelS, S(S), and S8), the most abundant cellulosome subunit, was undertaken to gain insight into its role in enzymatic and microbial cellulose solubilization. Cultures of the Cel48S deletion mutant (S mutant) were able to completely solubilize 10 g/L crystalline cellulose. The cellulose hydrolysis rate of the S mutant strain was 60% lower than the parent strain, with the S mutant strain also exhibiting a 40% reduction in cell yield. The cellulosome produced by the S mutant strain was purified by affinity digestion, characterized enzymatically, and found to have a 35% lower specific activity on Avicel. The composition of the purified cellulosome was analyzed by tandem mass spectrometry with APEX quantification and no significant changes in abundance were observed in any of the major (>1% of cellulosomal protein) enzymatic subunits. Although most cellulolytic bacteria have one family 48 cellulase, C. thermocellum has two, Cel48S and Cel48Y. Cellulose solubilization by a Cel48S and Cel48Y double knockout was essentially the same as that of the Cel48S single knockout. Our results indicate that solubilization of crystalline cellulose by C. thermocellum can proceed to completion without expression of a family 48 cellulase.
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