ArticleLab on a chip2010
Kinetics of NF-κB nucleocytoplasmic transport probed by single-cell screening without imaging.
Article in Lab on a chip, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 17 citations in OpenAlex.
- Recent Advances in Microscale Electroporation.Chemical reviews · 2022Review
- NanoSINC-seq dissects the isoform diversity in subcellular compartments of single cells.Science advances · 2021Article
- Single-cell analysis targeting the proteome.Nature reviews. Chemistry · 2020Review
- SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology.Genome biology · 2018Article
- Review
- Microfluidic electroporation for cellular analysis and delivery.Lab on a chip · 2013Review
- Controlled electroporation of the plasma membrane in microfluidic devices for single cell analysis.Biomicrofluidics · 2012Article
- Observing single cell NF-κB dynamics under stimulant concentration gradient.Analytical chemistry · 2012Article
- Micro total analysis systems for cell biology and biochemical assays.Analytical chemistry · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Transport of protein and RNA cargoes between the nucleus and cytoplasm (nucleocytoplasmic transport) is vital for a variety of cellular functions. The studies of kinetics involved in such processes have been hindered by the lack of quantitative tools for measurement of the nuclear and cytosolic fractions of an intracellular protein at the single cell level for a cell population. In this report, we describe using a novel method, microfluidic electroporative flow cytometry, to study kinetics of nucleocytoplasmic transport of an important transcription factor NF-κB. With data collected from single cells, we quantitatively characterize the population-averaged kinetic parameters such as the rate constants and apparent activation barrier for NF-κB transport. Our data demonstrate that NF-κB nucleocytoplasmic transport fits first-order kinetics very well and is a fairly reversible process governed by equilibrium thermodynamics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.