ArticleProceedings of the National Academy of Sciences of the United States of America2010
Inducible expression eliminates the fitness cost of vancomycin resistance in enterococci.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 122 citations in OpenAlex.
- Impacts of feeding preweaned calves milk containing drug residues on the functional profile of the fecal microbiota.Scientific reports · 2018Trial
- Pairwise Growth Dynamics of EnvironmentalAntibiotics (Basel, Switzerland) · 2026Article
- Fitness costs of Tn1546-type transposons harboring the vanA operon by plasmid type and structural diversity in Enterococcus faecium.Annals of clinical microbiology and antimicrobials · 2024Article
- Reversion to sensitivity explains limited transmission of resistance in a hospital pathogen.bioRxiv : the preprint server for biology · 2024Article
- Recent advances in nanoantibiotics against multidrug-resistant bacteria.Nanoscale advances · 2023Review
- Transcriptional Response of vanB Operon in Staphylococcus aureus Against Vancomycin and Teicoplanin Stress.Current microbiology · 2023Article
- Vancomycin Resistance inMicroorganisms · 2022Review
- Heterologous Expression Reveals Ancient Properties of Tei3—A VanS Ortholog from the Teicoplanin Producer Actinoplanes teichomyceticusInternational journal of molecular sciences · 2022Article
- Low levels of tetracyclines select for a mutation that prevents the evolution of high-level resistance to tigecycline.PLoS biology · 2022Article
- Amplification of the ChromosomalMicrobiology spectrum · 2022Article
- Multidrug-Resistant Bacteria: Their Mechanism of Action and Prophylaxis.BioMed research international · 2022Review
- Multidrug-Resistant Bacteria in the Community: An Update.Infectious disease clinics of North America · 2020Review
- Emergence ofAntibiotics (Basel, Switzerland) · 2020Article
- Lenzimycins A and B, Metabolites With Antibacterial Properties FromFrontiers in microbiology · 2020Article
- Fluorescent reporter plasmids for single-cell and bulk-level composition assays in E. faecalis.PloS one · 2020Article
- L,D-Transpeptidase Specific Probe Reveals Spatial Activity of Peptidoglycan Cross-Linking.ACS chemical biology · 2019Article
- Dissemination and genetic analysis of the stealthy vanB gene clusters of Enterococcus faecium clinical isolates in Japan.BMC microbiology · 2018Article
- Environmental pleiotropy and demographic history direct adaptation under antibiotic selection.Heredity · 2018Article
- Transferable vancomycin resistance in clade B commensal-type Enterococcus faecium.The Journal of antimicrobial chemotherapy · 2018Article
- Prediction of antibiotic resistance: time for a new preclinical paradigm?Nature reviews. Microbiology · 2017Review
2 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inducible vancomycin resistance in enterococci is due to a sophisticated mechanism that combines synthesis of cell wall peptidoglycan precursors with low affinity for glycopeptides and elimination of the normal target precursors. Although this dual mechanism, which involves seven genes organized in two operons, is predicted to have a high fitness cost, resistant enterococci have disseminated worldwide. We have evaluated the biological cost of VanB-type resistance due to acquisition of conjugative transposon Tn1549 in Enterococcus faecium and Enterococcus faecalis. Because fitness was dependent on the integration site of Tn1549, an isogenic set of E. faecalis was constructed to determine the cost of inducible or constitutive expression of resistance or of carriage of Tn1549. A luciferase gene was inserted in the integrase gene of the transposon to allow differential quantification of the strains in cocultures and in the digestive tract of gnotobiotic mice. Both in vitro and in vivo, carriage of inactivated or inducible Tn1549 had no cost for the host in the absence of induction by vancomycin. In contrast, induced or constitutively resistant strains not only had reduced fitness but were severely impaired in colonization ability and dissemination among mice. These data indicate that tight regulation of resistance expression drastically reduces the biological cost associated with vancomycin resistance in Enterococcus spp. and accounts for the widespread dissemination of these strains. Our findings are in agreement with the observation that regulation of expression is common in horizontally acquired resistance and represents an efficient evolutionary pathway for resistance determinants to become selectively neutral.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.