ArticleThe Journal of endocrinology2010
Ex3αERKO male infertility phenotype recapitulates the αERKO male phenotype.
Article in The Journal of endocrinology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- Molecular Pathways Implicated in the Differentiation and Function of Epididymal Basal Cells.Advances in experimental medicine and biology · 2025Review
- Role for Nongenomic Estrogen Signaling in Male Fertility.Endocrinology · 2024Article
- Lrh1 can help reprogram sexual cell fate and is required for Sertoli cell development and spermatogenesis in the mouse testis.PLoS genetics · 2022Article
- A framework for high-resolution phenotyping of candidate male infertility mutants: from human to mouse.Human genetics · 2021Review
- The sex-specific role of p38 MAP kinase in CNS autoimmunity is regulated by estrogen receptor alpha.Journal of neuroimmunology · 2020Article
- Estrogens in Human Male Gonadotropin Secretion and Testicular Physiology From Infancy to Late Puberty.Frontiers in endocrinology · 2020Review
- Estrogens in Male Physiology.Physiological reviews · 2017Review
- ESR1 inhibits hCG-induced steroidogenesis and proliferation of progenitor Leydig cells in mice.Scientific reports · 2017Article
- Interactions between oestrogen and 1α,25(OH)Basic and clinical andrology · 2017Article
- Membrane-Localized Estrogen Receptor 1 Is Required for Normal Male Reproductive Development and Function in Mice.Endocrinology · 2016Article
- Distinct functions and regulation of epithelial progesterone receptor in the mouse cervix, vagina, and uterus.Oncotarget · 2016Article
- Oestrogen action and male fertility: experimental and clinical findings.Cellular and molecular life sciences : CMLS · 2015Review
- Generation of Esr1-knockout rats using zinc finger nuclease-mediated genome editing.Endocrinology · 2014Article
- Estrogen hormone physiology: reproductive findings from estrogen receptor mutant mice.Reproductive biology · 2014Review
- The Transactivating Function 2 (AF-2) of Estrogen Receptor (ER) α is Indispensable for ERα-mediated Physiological Responses and AF-1 Activity.Open journal of endocrine and metabolic diseases · 2013Article
- Transactivating function (AF) 2-mediated AF-1 activity of estrogen receptor α is crucial to maintain male reproductive tract function.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Overexpression of follistatin in the mouse epididymis disrupts fluid resorption and sperm transit in testicular excurrent ducts.Biology of reproduction · 2012Article
- Anabolic androgenic steroid abuse: multiple mechanisms of regulation of GABAergic synapses in neuroendocrine control regions of the rodent forebrain.Journal of neuroendocrinology · 2012Review
- Estrogen, efferent ductules, and the epididymis.Biology of reproduction · 2011Review
- Estrogens and development of the rete testis, efferent ductules, epididymis and vas deferens.Differentiation; research in biological diversityArticle
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Disruption of the Esr1 gene encoding estrogen receptor α (ERα) by insertion of a neomycin resistance gene (neo) into exon 2 (αERKO mice) was shown previously to cause infertility in male mice. While full-length ERα protein was not expressed in αERKO mice, alternative splicing resulted in the low-level expression of a truncated form lacking the N-terminus A/B domain and containing the DNA- and ligand-binding domains. Thus, it was unclear whether the reproductive phenotype in αERKO males was only due to the lack of full-length ERα or was affected by the presence of the variant ERα isoform. The present study examined male mice with deletion of exon 3 of Esr1 gene, lacking the DNA-binding domain, and null for ERα (Ex3αERKO). Dilation of some seminiferous tubules was apparent in male Ex3αERKO mice as early as postnatal day 10 and was pronounced in all tubules from day 20 onward. At 6 weeks of age, sperm numbers and sperm motility were lower in Ex3αERKO mice than in wild-type (WT) mice, and the rete testis and efferent ductules were dilated. Mating studies determined that adult Ex3αERKO males were infertile and failed to produce copulatory plugs. Serum testosterone levels and Hsd17b3 and Cyp17a1 transcript levels were significantly higher, but serum estradiol, progesterone, LH, and FSH levels and Cyp19a1 transcript levels were not significantly different from those in WT mice. These results confirm and extend those seen in other studies on male mice with deletion of exon 3 of Esr1 gene. In addition, the reproductive phenotype of male Ex3αERKO mice recapitulated the phenotype of αERKO mice, strongly suggesting that the αERKO male infertility was not due to the presence of the DNA-binding domain in the truncated form of ERα and that full-length ERα is essential for maintenance of male fertility.
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