Evidence map›Paper›PMID 20833003›Full record

ArticleColloids and surfaces. B, Biointerfaces2011

Staining proteins: a simple method to increase the sensitivity of ellipsometric measurements in adsorption studies.

M Reza Nejadnik, Carlos D Garcia

Abstract read
In one paragraph

Article in Colloids and surfaces. B, Biointerfaces, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
4.7field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 20 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

M Reza NejadnikDepartment of Chemistry, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA.
Carlos D Garcia
The University of Texas at San Antonio · US

Funding

UNDERSTANDING THE INTERACTION OF ANKYRINS AND NANOMATERIALSSC3GM081085 · NIGMS · UNIVERSITY OF TEXAS SAN ANTONIO · PI GARCIA, CARLOS DIEGO · 2007 to 2015
$844k
NIGMS NIH HHS 1SC3GM081085NIGMS NIH HHS SC3 GM081085
6 · The paper itself

Abstract

This communication describes a simple way to improve the sensitivity of spectroscopic ellipsometry, when applied to monitor the adsorption of proteins to solid surfaces. The method described herein is based on the reaction of a commercially available dye (Coomassie brilliant blue G) with the adsorbed proteins and the subsequent analysis by spectroscopic ellipsometry. In order to demonstrate the potential advantages of this method, the adsorption of bovine serum albumin to an antifouling coating was also investigated. According to our results, the modification with the dye significantly affects the optical properties of the adsorbed protein layer, which can be represented using a simple optical model (Lorentz). In general, the proposed modification increases the sensitivity of the detection by 2.5 ± 0.4-fold and enables the analysis of thin layers of adsorbed protein not obtainable by conventional methods. These results particularly reveal the importance of the proposed modification for the evaluation of low adsorbing substrates and antifouling coatings.

Indexed as

AdsorptionAnimalsCattleModels, BiologicalOptics and PhotonicsPolymersProteinsSerum Albumin, BovineSpectrum AnalysisStaining and LabelingSubstrate SpecificityTime FactorsPolymersProteinsSerum Albumin, Bovine

Identifiers

PMID20833003
PMCPMC2952718
OpenAlexW2082790017

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.