ArticleColloids and surfaces. B, Biointerfaces2011
Staining proteins: a simple method to increase the sensitivity of ellipsometric measurements in adsorption studies.
Article in Colloids and surfaces. B, Biointerfaces, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Who cites it
10 citing papers in PubMed, 20 citations in OpenAlex.
- A Tale of Two Stressors in Biologic Drug Product Development: Shaking Mode and Primary Packaging.Pharmaceutical research · 2026Article
- Electrode Potential-Dependent Studies of Protein Adsorption on TiMolecules (Basel, Switzerland) · 2023Article
- Electrochemically Preadsorbed Collagen Promotes Adult Human Mesenchymal Stem Cell Adhesion.Tissue engineering. Part C, Methods · 2016Article
- Protein adsorption onto nanomaterials for the development of biosensors and analytical devices: a review.Analytica chimica acta · 2015Review
- Immobilization of glucose oxidase to nanostructured films of polystyrene-block-poly(2-vinylpyridine).Journal of colloid and interface science · 2014Article
- Potential-assisted adsorption of bovine serum albumin onto optically transparent carbon electrodes.Langmuir : the ACS journal of surfaces and colloids · 2013Article
- Ultrathin optically transparent carbon electrodes produced from layers of adsorbed proteins.Langmuir : the ACS journal of surfaces and colloids · 2013Article
- Research Spotlight: The next big thing is actually small.Bioanalysis · 2012Article
- Nanomolar Detection of Glutamate at a Biosensor Based on Screen-Printed Electrodes Modified with Carbon Nanotubes.Electroanalysis · 2011Article
- Adsorption of Proteins to Thin-Films of PDMS and Its Effect on the Adhesion of Human Endothelial Cells.RSC advances · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
This communication describes a simple way to improve the sensitivity of spectroscopic ellipsometry, when applied to monitor the adsorption of proteins to solid surfaces. The method described herein is based on the reaction of a commercially available dye (Coomassie brilliant blue G) with the adsorbed proteins and the subsequent analysis by spectroscopic ellipsometry. In order to demonstrate the potential advantages of this method, the adsorption of bovine serum albumin to an antifouling coating was also investigated. According to our results, the modification with the dye significantly affects the optical properties of the adsorbed protein layer, which can be represented using a simple optical model (Lorentz). In general, the proposed modification increases the sensitivity of the detection by 2.5 ± 0.4-fold and enables the analysis of thin layers of adsorbed protein not obtainable by conventional methods. These results particularly reveal the importance of the proposed modification for the evaluation of low adsorbing substrates and antifouling coatings.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.