ArticleBiomaterials2010
Using growth factor arrays and micropatterned co-cultures to induce hepatic differentiation of embryonic stem cells.
Article in Biomaterials, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 64 citations in OpenAlex.
- Article
- A Microfluidic Device for Long-Term Maintenance of Organotypic Liver Cultures.Advanced materials technologies · 2023Article
- Insight and Recent Advances into the Role of Topography on the Cell Differentiation and Proliferation on Biopolymeric Surfaces.International journal of molecular sciences · 2022Review
- The Role of the Microenvironment in Controlling the Fate of Bioprinted Stem Cells.Chemical reviews · 2020Review
- Machine learning metrology of cell confinement in melt electrowritten three-dimensional biomaterial substrates.Microsystems & nanoengineering · 2019Article
- Current Perspectives Regarding Stem Cell-Based Therapy for Liver Cirrhosis.Canadian journal of gastroenterology & hepatology · 2018Review
- 3D bioprinting using stem cells.Pediatric research · 2018Review
- Induction of hepatocyte-like cells from human umbilical cord-derived mesenchymal stem cells by defined microRNAs.Journal of cellular and molecular medicine · 2017Article
- The Use of Induced Pluripotent Stem Cells for the Study and Treatment of Liver Diseases.Current protocols in toxicology · 2016Review
- In vitro culture of isolated primary hepatocytes and stem cell-derived hepatocyte-like cells for liver regeneration.Protein & cell · 2015Review
- Drug-eluting microarrays to identify effective chemotherapeutic combinations targeting patient-derived cancer stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Synthetic Extracellular Microenvironment for Modulating Stem Cell Behaviors.Biomarker insights · 2015Review
- Multilayered heparin hydrogel microwells for cultivation of primary hepatocytes.Advanced healthcare materials · 2014Article
- Local control of hepatic phenotype with growth factor-encoded surfaces.Integrative biology : quantitative biosciences from nano to macro · 2014Article
- Engineering the human pluripotent stem cell microenvironment to direct cell fate.Biotechnology advances · 2013Review
- Promotion of hepatic differentiation of bone marrow mesenchymal stem cells on decellularized cell-deposited extracellular matrix.BioMed research international · 2013Article
- Generation of functional hepatic cells from pluripotent stem cells.Journal of stem cell research & therapy · 2012Article
- Life on a microarray: assessing live cell functions in a microarray format.Cellular and molecular life sciences : CMLS · 2012Review
- Probing embryonic stem cell autocrine and paracrine signaling using microfluidics.Annual review of analytical chemistry (Palo Alto, Calif.) · 2012Review
- The knee meniscus: structure-function, pathophysiology, current repair techniques, and prospects for regeneration.Biomaterials · 2011Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The success in driving embryonic stem cells towards hepatic lineage has been confounded by the complexity and cost of differentiation protocols that employ large quantities of expensive growth factors (GFs). Instead of supplementing culture media with soluble GFs, we investigated cultivation and differentiation of mouse embryonic stem cells (mESCs) on printed arrays of GFs. Hepatocyte growth factor (HGF), basic fibroblast growth factor (bFGF) and bone morphogenetic protein (BMP4) were mixed in solution with fibronectin and collagen (I) and then printed onto silane-modified glass slides to form 500 μm diameter protein spots. mESCs were cultured on top of GF spots for up to 12 days and analyzed by RT-PCR and immunostaining at different time points. The stem cells residing on HGF-containing combinations of GFs exhibited requisite features of hepatic differentiation including pronounced loss in pluripotency (Oct4), transient (up and down) expression of endoderm (Sox17) and upregulation of early hepatic markers--albumin and alpha-fetoprotein. The hepatic differentiation was enhanced further by adding hepatic stellate cells to surfaces that already contained mESCs on GF spots. A combination of co-culture with non-parenchymal liver cells and the optimal GF stimulation was found to induce endoderm and hepatic phenotype earlier and to a much greater extent than the GF arrays or micropatterned co-cultures used individually. While this paper investigated hepatic differentiation of mouse ESCs, our findings and stem cell culture approaches are likely to be relevant for human ESC cultivation. Overall, the platform combining printed GF arrays and heterotypic co-cultures will be broadly applicable for identifying the composition of the microenvironment niche for ESC differentiation into various tissue types.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.