ArticleThe Journal of biological chemistry2010
A eukaryotic (insect) tricistronic mRNA encodes three proteins selected by context-dependent scanning.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 20 citations in OpenAlex.
- Dark matter matters: from noncoding RNA regulation to cancer immunity.Oncoimmunology · 2026Review
- Broad-complex transcription factor mediates opposing hormonal regulation of two phylogenetically distant arginine kinase genes in Tribolium castaneum.Communications biology · 2020Article
- A possible mechanism for low affinity of silkworm NaJournal of bioenergetics and biomembranes · 2017Article
- Molecular mechanism underlying juvenile hormone-mediated repression of precocious larval-adult metamorphosis.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Solution Structure and Expression Profile of an Insect Cytokine: Manduca sexta Stress Response Peptide-2.Protein and peptide letters · 2017Article
- Krüppel Homolog 1 Inhibits Insect Metamorphosis via Direct Transcriptional Repression of Broad-Complex, a Pupal Specifier Gene.The Journal of biological chemistry · 2016Article
- Death of a dogma: eukaryotic mRNAs can code for more than one protein.Nucleic acids research · 2016Review
- A role for Taiman in insect metamorphosis.PLoS genetics · 2014Article
- Effect of ATG initiation codon context motifs on the efficiency of translation of mRNA derived from exogenous genes in the transgenic silkworm, Bombyx mori.SpringerPlus · 2014Article
- The melanoma antigens MELOE-1 and MELOE-2 are translated from a bona fide polycistronic mRNA containing functional IRES sequences.PloS one · 2013Article
- Establishment of a versatile cell line for juvenile hormone signaling analysis in Tribolium castaneum.Scientific reports · 2013Article
- Transcriptional regulation of juvenile hormone-mediated induction of Krüppel homolog 1, a repressor of insect metamorphosis.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Fungal ecdysteroid-22-oxidase, a new tool for manipulating ecdysteroid signaling and insect development.The Journal of biological chemistry · 2012Article
- Pyrosequencing-based expression profiling and identification of differentially regulated genes from Manduca sexta, a lepidopteran model insect.Insect biochemistry and molecular biology · 2011Article
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8 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Eukaryotic mRNAs are generally considered monocistronic and encode only one protein. Although dicistronic mRNAs encoding two proteins were found in fungi, plants, and animals, polycistronic mRNAs encoding more than two proteins have remained elusive so far in any eukaryote. Here we demonstrate that a single mRNA from silkworm encodes the precursor of an insect cytokine paralytic peptide (PP) and two new cytokine precursor-like proteins, uENF1 and uENF2. RT-PCR analysis showed that this mRNA is widely conserved in moths. Western blot analyses and reporter assays using its modified mRNAs, created by replacing each one of the three ORFs with the firefly luciferase ORF, showed that all three proteins were translated from this mRNA in cell lines, larval tissues, and cell-free systems. Insertion experiments using the Renilla luciferase ORF or a stem loop ruled out the possible involvement of internal ribosome entry site in the three protein translation. On the other hand, systematic mutation analysis of the translation initiation sequence of the 5'-proximal uENF1 ORF suggested that the context-dependent leaky-scanning mechanism is involved in translation of the downstream uENF2 and PP ORFs. In vitro, a synthetic peptide corresponding to the putative mature form of uENF1 stimulated spreading of hemocytes as did the synthetic PP, whereas that of uENF2 antagonized the stimulating activities of PP and the uENF1 peptide, suggesting that the three proteins control cellular immunity interactively. Thus, eukaryotes have a cellular tricistronic mRNA that encodes three functionally related proteins as in an operon.
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