ArticleMicrobiology (Reading, England)2010
6S RNA regulation of relA alters ppGpp levels in early stationary phase.
Article in Microbiology (Reading, England), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- RIP-seq reveals RNAs that interact with RNA polymerase and primary sigma factors in bacteria.Nucleic acids research · 2024Article
- Probing the conformational changes ofFrontiers in molecular biosciences · 2023Article
- 6S RNA-Dependent Susceptibility to RNA Polymerase Inhibitors.Antimicrobial agents and chemotherapy · 2022Article
- Involvement ofInternational journal of molecular sciences · 2022Article
- NT-CRISPR, combining natural transformation and CRISPR-Cas9 counterselection for markerless and scarless genome editing in Vibrio natriegens.Communications biology · 2022Article
- Dynamic Metabolic Response to (p)ppGpp Accumulation inFrontiers in microbiology · 2022Article
- Cellular Self-Digestion and Persistence in Bacteria.Microorganisms · 2021Review
- Gene Regulation and Transcriptomics.Current issues in molecular biology · 2021Article
- 6S-2 RNA deletion in the undomesticatedRNA biology · 2021Article
- Characterization of 6S RNA in the Lyme disease spirochete.Molecular microbiology · 2020Article
- Regulation of Global Transcription inG3 (Bethesda, Md.) · 2018Article
- 6S RNA, a Global Regulator of Transcription.Microbiology spectrum · 2018Review
- The Stringent Response-Regulated sRNA Transcriptome ofFrontiers in cellular and infection microbiology · 2018Article
- 6S RNA in Rhodobacter sphaeroides: 6S RNA and pRNA transcript levels peak in late exponential phase and gene deletion causes a high salt stress phenotype.RNA biology · 2017Article
- Molecular Basis of Stationary Phase Survival and Applications.Frontiers in microbiology · 2017Review
- A computational strategy for the search of regulatory small RNAs in Actinobacillus pleuropneumoniae.RNA (New York, N.Y.) · 2016Article
- Maturation of 6S regulatory RNA to a highly elongated structure.The FEBS journal · 2015Article
- Identification of novel small RNAs and characterization of the 6S RNA of Coxiella burnetii.PloS one · 2014Article
- Regulation of transcription by 6S RNAs: insights from the Escherichia coli and Bacillus subtilis model systems.RNA biology · 2014Review
- 6S-1 RNA function leads to a delay in sporulation in Bacillus subtilis.Journal of bacteriology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
6S RNA is a small, non-coding RNA that interacts directly with σ(70)-RNA polymerase and regulates transcription at many σ(70)-dependent promoters. Here, we demonstrate that 6S RNA regulates transcription of relA, which encodes a ppGpp synthase. The 6S RNA-dependent regulation of relA expression results in increased ppGpp levels during early stationary phase in cells lacking 6S RNA. These changes in ppGpp levels, although modest, are sufficient to result in altered regulation of transcription from σ(70)-dependent promoters sensitive to ppGpp, including those promoting expression of genes involved in amino acid biosynthesis and rRNA. These data place 6S RNA as another player in maintaining appropriate gene expression as cells transition into stationary phase. Independent of this ppGpp-mediated 6S RNA-dependent regulation, we also demonstrate that in later stationary phase, 6S RNA continues to downregulate transcription in general, and specifically at a subset of the amino acid promoters, but through a mechanism that is independent of ppGpp and which we hypothesize is through direct regulation. In addition, 6S RNA-dependent regulation of σ(S) activity is not mediated through observed changes in ppGpp levels. We suggest a role for 6S RNA in modulating transcription of several global regulators directly, including relA, to downregulate expression of key pathways in response to changing environmental conditions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.