ArticleStem cells (Dayton, Ohio)2010
Functional control of transplantable human ESC-derived neurons via optogenetic targeting.
Article in Stem cells (Dayton, Ohio), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed, 87 citations in OpenAlex.
- Higher Purity of Phosphatidylserine Improves Human Cortical Neuron Function by Modulating SIRT1-PGC-1α Pathways.Brain sciences · 2026Article
- Effects of optogenetic stimulation on neural gene expression in human bone marrow and adipose-derived mesenchymal stem cells.BMC biotechnology · 2025Article
- Towards translational optogenetics.Nature biomedical engineering · 2023Review
- Optogenetic Control of Human Stem Cell-Derived Neurons.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Application of Optogenetics for Muscle Cells and Stem Cells.Advances in experimental medicine and biology · 2021Article
- Optogenetic Modulation of Neural Progenitor Cells Improves Neuroregenerative Potential.International journal of molecular sciences · 2020Article
- BrainPhys neuronal medium optimized for imaging and optogenetics in vitro.Nature communications · 2020Article
- Modulating electrophysiology of motor neural networks via optogenetic stimulation during neurogenesis and synaptogenesis.Scientific reports · 2020Article
- Bone Regeneration Induced by Strontium Folate Loaded Biohybrid Scaffolds.Molecules (Basel, Switzerland) · 2019Review
- Optogenetic control of iPS cell-derived neurons in 2D and 3D culture systems using channelrhodopsin-2 expression driven by the synapsin-1 and calcium-calmodulin kinase II promoters.Journal of tissue engineering and regenerative medicine · 2019Article
- Optogenetic stimulation inhibits the self-renewal of mouse embryonic stem cells.Cell & bioscience · 2019Article
- Article
- Human Models Are Needed for Studying Human Neurodevelopmental Disorders.American journal of human genetics · 2018Review
- Article
- On-demand optogenetic activation of human stem-cell-derived neurons.Scientific reports · 2017Article
- Stem cell transplantation therapy for multifaceted therapeutic benefits after stroke.Progress in neurobiology · 2017Review
- Genetically Encoded Photoactuators and Photosensors for Characterization and Manipulation of Pluripotent Stem Cells.Theranostics · 2017Review
- Predicting the functional states of human iPSC-derived neurons with single-cell RNA-seq and electrophysiology.Molecular psychiatry · 2016Article
- Microfluidic device for the formation of optically excitable, three-dimensional, compartmentalized motor units.Science advances · 2016Article
- hESC-derived neural progenitors prevent xenograft rejection through neonatal desensitisation.Experimental neurology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Current methods to examine and regulate the functional integration and plasticity of human ESC (hESC)-derived neurons are cumbersome and technically challenging. Here, we engineered hESCs and their derivatives to express the light-gated channelrhodopsin-2 (ChR2) protein to overcome these deficiencies. Optogenetic targeting of hESC-derived neurons with ChR2 linked to the mCherry fluorophore allowed reliable cell tracking as well as light-induced spiking at physiological frequencies. Optically induced excitatory and inhibitory postsynaptic currents could be elicited in either ChR2(+) or ChR2(-) neurons in vitro and in acute brain slices taken from transplanted severe combined immunodeficient (SCID) mice. Furthermore, we created a clonal hESC line that expresses ChR2-mCherry under the control of the synapsin-1 promoter. On neuronal differentiation, ChR2-mCherry expression was restricted to neurons and was stably expressed for at least 6 months, providing more predictable light-induced currents than transient infections. This pluripotent cell line will allow both in vitro and in vivo analysis of functional development as well as the integration capacity of neuronal populations for cell-replacement strategies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.