ArticleJournal of assisted reproduction and genetics2010
Dicer is a key player in oocyte maturation.
Article in Journal of assisted reproduction and genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 59 citations in OpenAlex.
- Oocyte Maturation and miRNAs: Studying a Complicate Interaction to Reveal Possible Biomarkers for Female Infertility.Diseases (Basel, Switzerland) · 2024Review
- Evaluation of the expression level of microRNA-21, microRNA-15a, microRNA-372 in human follicular fluid stem cells-derived oocyte-like cells (OLCs).JBRA assisted reproduction · 2024Article
- Article
- Comparison of miRNA landscapes between the human oocytes with or without arrested development.Journal of assisted reproduction and genetics · 2022Article
- Processing ofRNA (New York, N.Y.) · 2020Article
- Multi-omics analysis reveals that natural hibernation is crucial for oocyte maturation in the female Chinese alligator.BMC genomics · 2020Article
- MiR-423-5p may regulate ovarian response to ovulation induction via CSF1.Reproductive biology and endocrinology : RB&E · 2020Article
- Roles of microRNAs in mammalian reproduction: from the commitment of germ cells to peri-implantation embryos.Biological reviews of the Cambridge Philosophical Society · 2019Review
- Constitutive Dicer1 phosphorylation accelerates metabolism and aging in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- New roles for Dicer in the nucleolus and its relevance to cancer.Cell cycle (Georgetown, Tex.) · 2017Review
- Epigenetics: A key paradigm in reproductive health.Clinical and experimental reproductive medicine · 2016Review
- Gonadotrope-specific deletion of Dicer results in severely suppressed gonadotropins and fertility defects.The Journal of biological chemistry · 2015Article
- shRNA off-target effects in vivo: impaired endogenous siRNA expression and spermatogenic defects.PloS one · 2015Article
- Article
- Integrating microRNAs into the complexity of gonadotropin signaling networks.Frontiers in cell and developmental biology · 2013Review
- Minireview: The roles of small RNA pathways in reproductive medicine.Molecular endocrinology (Baltimore, Md.) · 2011Review
- Differentially expressed micoRNAs in human oocytes.Journal of assisted reproduction and genetics · 2011Article
- A focused evaluation of genetic and epigenetic contributions to common infertility conditions.Therapeutic advances in reproductive healthReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveApply Dicer siRNA to study functions of Dicer and miRNA during oogenesis. MATERIALS AND
methodsMouse oocytes were injected with Dicer siRNA and negative control siRNA and then matured in vitro. After IVM, oocytes were examined for maturation rates, spindle and chromosomal organization, and various gene expressions.
resultsDicer siRNA significantly reduced maturation rates, increased abnormal spindle and chromosomal organization, and reduced the transcripts of Dicer miRNAs, spindle formation proteins (plk1 and AURKA) and spindle check points (Bub1, Bublb). Depletion of bulb16 markedly prohibited the first polar body extrusion and increased the incidence of misaligned chromosomes and abnormal meiotic spindle assembly.
conclusionDicer siRNA triggered a cascade reduction for gene expressions starting from Dicer to miRNAs than to spindle assembly proteins and checkpoints which led to abnormal spindle and chromosomal organization. Thus, Dicer and miRNA appeared to play an important role during oogenesis and were essential for meiotic completion.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.