ArticleThe Journal of biological chemistry2010
Biochemical characterization of the transcriptional regulator BzdR from Azoarcus sp. CIB.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 33 citations in OpenAlex.
- Article
- Responsiveness of Aromatoleum aromaticum EbN1Applied and environmental microbiology · 2021Article
- Towards Exploring Toxin-Antitoxin Systems inInternational journal of molecular sciences · 2019Article
- Further Insights into the Architecture of theGenes · 2019Article
- Article
- Molecular and Functional Insights into the Regulation of d-Galactonate Metabolism by the Transcriptional Regulator DgoR inJournal of bacteriology · 2019Article
- The osnR gene of Corynebacterium glutamicum plays a negative regulatory role in oxidative stress responses.Journal of industrial microbiology & biotechnology · 2019Article
- Comparative Genomic Analysis of the Regulation of Aromatic Metabolism in Betaproteobacteria.Frontiers in microbiology · 2019Article
- Article
- Engineering a bzd cassette for the anaerobic bioconversion of aromatic compounds.Microbial biotechnology · 2017Article
- Refactoring the λ phage lytic/lysogenic decision with a synthetic regulator.MicrobiologyOpen · 2016Article
- Structural and functional analysis of the Rpf2-Rrs1 complex in ribosome biogenesis.Nucleic acids research · 2015Article
- Unraveling the specific regulation of the central pathway for anaerobic degradation of 3-methylbenzoate.The Journal of biological chemistry · 2015Article
- Benzoate mediates the simultaneous repression of anaerobic 4-methylbenzoate and succinate utilization in Magnetospirillum sp. strain pMbN1.BMC microbiology · 2014Article
- AccR is a master regulator involved in carbon catabolite repression of the anaerobic catabolism of aromatic compounds in Azoarcus sp. CIB.The Journal of biological chemistry · 2014Article
- Identification of a missing link in the evolution of an enzyme into a transcriptional regulator.PloS one · 2013Article
- Bacterial degradation of benzoate: cross-regulation between aerobic and anaerobic pathways.The Journal of biological chemistry · 2012Article
- Benzoate mediates repression of C(4)-dicarboxylate utilization in "Aromatoleum aromaticum" EbN1.Journal of bacteriology · 2012Article
- Microbial degradation of aromatic compounds - from one strategy to four.Nature reviews. Microbiology · 2011Review
- Dynamic allostery: linkers are not merely flexible.Structure (London, England : 1993) · 2011Review
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The BzdR transcriptional regulator that controls the P(N) promoter responsible for the anaerobic catabolism of benzoate in Azoarcus sp. CIB constitutes the prototype of a new subfamily of transcriptional regulators. Here, we provide some insights about the functional-structural relationships of the BzdR protein. Analytical ultracentrifugation studies revealed that BzdR is homodimeric in solution. An electron microscopy three-dimensional reconstruction of the BzdR dimer has been obtained, and the predicted structures of the respective N- and C-terminal domains of each BzdR monomer could be fitted into such a reconstruction. Gel retardation and ultracentrifugation experiments have shown that the binding of BzdR to its cognate promoter is cooperative. Different biochemical approaches revealed that the effector molecule benzoyl-CoA induces conformational changes in BzdR without affecting its oligomeric state. The BzdR-dependent inhibition of the P(N) promoter and its activation in the presence of benzoyl-CoA have been established by in vitro transcription assays. The monomeric BzdR4 and BzdR5 mutant regulators revealed that dimerization of BzdR is essential for DNA binding. Remarkably, a BzdRΔL protein lacking the linker region connecting the N- and C-terminal domains of BzdR is also dimeric and behaves as a super-repressor of the P(N) promoter. These data suggest that the linker region of BzdR is not essential for protein dimerization, but rather it is required to transfer the conformational changes induced by the benzoyl-CoA to the DNA binding domain leading to the release of the repressor. A model of action of the BzdR regulator has been proposed.
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