ArticleThe Journal of biological chemistry2011
Structural studies and the assembly of the heptameric post-translational translocon complex.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 40 citations in OpenAlex.
- A kalihinol analog disrupts apicoplast function and vesicular trafficking inScience (New York, N.Y.) · 2024Article
- The GET pathway is a major bottleneck for maintaining proteostasis in Saccharomyces cerevisiae.Scientific reports · 2023Article
- Emerging View on the Molecular Functions of Sec62 and Sec63 in Protein Translocation.International journal of molecular sciences · 2021Review
- How does Sec63 affect the conformation of Sec61 in yeast?PLoS computational biology · 2021Article
- Overlapping function of Hrd1 and Ste24 in translocon quality control provides robust channel surveillance.The Journal of biological chemistry · 2020Article
- Membrane phospholipid alteration causes chronic ER stress through early degradation of homeostatic ER-resident proteins.Scientific reports · 2019Article
- Two alternative binding mechanisms connect the protein translocation Sec71-Sec72 complex with heat shock proteins.The Journal of biological chemistry · 2017Article
- The SND proteins constitute an alternative targeting route to the endoplasmic reticulum.Nature · 2016Article
- Analysis of membrane proteins localizing to the inner nuclear envelope in living cells.The Journal of cell biology · 2016Article
- Article
- Mammalian SRP receptor switches the Sec61 translocase from Sec62 to SRP-dependent translocation.Nature communications · 2015Article
- Protein O-mannosyltransferases associate with the translocon to modify translocating polypeptide chains.The Journal of biological chemistry · 2014Article
- AtTPR7 as part of the Arabidopsis Sec post-translocon.Plant signaling & behavior · 2013Article
- Protein translocation across the rough endoplasmic reticulum.Cold Spring Harbor perspectives in biology · 2013Review
- Article
- Chaperone receptors: guiding proteins to intracellular compartments.Protoplasma · 2012Review
- Role of human sec63 in modulating the steady-state levels of multi-spanning membrane proteins.PloS one · 2012Article
- An EGF-like protein forms a complex with PfRh5 and is required for invasion of human erythrocytes by Plasmodium falciparum.PLoS pathogens · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
In Saccharomyces cerevisiae, some of the nascent chains can be post-translationally translocated into the endoplasmic reticulum through the heptameric post-translational translocon complex (post-translocon). This membrane-protein complex is composed of the protein-conducting channel and the tetrameric Sec62/63 complex. The Sec62/63 complex plays crucial roles in targeting of the signal recognition particle-independent protein substrate to the protein-conducting channel and in assembly of the post-translocon. Although the molecular mechanism of the post-translational translocation process has been well established, the structure of the post-translocon and how the channel and the Sec62/63 complex form the heptameric complex are largely uncharacterized. Here, we report a 20-Å resolution cryo-electron microscopy structure of the post-translocon. The purified post-translocon was found to have a mass of 287 kDa, which is consistent with the unit stoichiometry of the seven subunits as determined by a cysteine labeling experiment. We demonstrated that Triton X-100 dissociated the heptameric complex into three subcomplexes identified as the trimeric translocon Sec61-Sbh1-Sss1, the Sec63-Sec71-Sec72 trimer, and the heterotetramer Sec62-Sec63-Sec71-Sec72, respectively. Additionally, a role of the sixth cytosolic loop of Sec61 in assembly of the post-translocon was demonstrated. Mutations of conserved, positively charged amino acid residues in the loop caused decreased formation of the post-translocon. These studies provide the first architectural description of the yeast post-translocon.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.