ArticleThe Journal of biological chemistry2010
Heterogeneity of phosphatidic acid levels and distribution at the plasma membrane in living cells as visualized by a Föster resonance energy transfer (FRET) biosensor.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 46 citations in OpenAlex.
- Phospholipase D2 acts upstream of PI4P 5-kinases in the response of neurons to extracellular viscosity.iScience · 2026Article
- AGPAT2 acts at the crossroads of lipid biosynthesis and DRP1-mediated ER morphogenesis.Nature communications · 2025Article
- PILS-Nir1 is a sensitive phosphatidic acid biosensor that reveals mechanisms of lipid production.The Journal of cell biology · 2025Article
- PILS-Nir1 is a novel phosphatidic acid biosensor that reveals mechanisms of lipid production.bioRxiv : the preprint server for biology · 2025Article
- Phosphatidic acid signaling and function in nuclei.Progress in lipid research · 2024Review
- Dynamic responses of PA to environmental stimuli imaged by a genetically encoded mobilizable fluorescent sensor.Plant communications · 2023Article
- Identification of Key Phospholipids That Bind and Activate Atypical PKCs.Biomedicines · 2021Article
- Lipid Phosphate Phosphatases and Cancer.Biomolecules · 2020Review
- Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.Chemical reviews · 2018Review
- Synergistic Ensemble of Optogenetic Actuators and Dynamic Indicators in Cell Biology.Molecules and cells · 2018Review
- Functional link between plasma membrane spatiotemporal dynamics, cancer biology, and dietary membrane-altering agents.Cancer metastasis reviews · 2018Review
- Quantifying lipid changes in various membrane compartments using lipid binding protein domains.Cell calcium · 2017Review
- Measuring Phospholipase D Enzymatic Activity Through Biochemical and Imaging Methods.Methods in enzymology · 2017Article
- Guidelines for the Use of Protein Domains in Acidic Phospholipid Imaging.Methods in molecular biology (Clifton, N.J.) · 2016Review
- Diacylglycerol Kinases: Shaping Diacylglycerol and Phosphatidic Acid Gradients to Control Cell Polarity.Frontiers in cell and developmental biology · 2016Review
- Lipid phosphate phosphatases and their roles in mammalian physiology and pathology.Journal of lipid research · 2015Review
- Effects of oral phosphatidic acid feeding with or without whey protein on muscle protein synthesis and anabolic signaling in rodent skeletal muscle.Journal of the International Society of Sports Nutrition · 2015Article
- Development of a Novel Tetravalent Synthetic Peptide That Binds to Phosphatidic Acid.PloS one · 2015Article
- Cellular and physiological roles for phospholipase D1 in cancer.The Journal of biological chemistry · 2014Review
- Temporal production of the signaling lipid phosphatidic acid by phospholipase D2 determines the output of extracellular signal-regulated kinase signaling in cancer cells.Molecular and cellular biology · 2014Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Phosphatidic acid (PA) is one of the major phospholipids in the plasma membrane. Although it has been reported that PA plays key roles in cell survival and morphology, it remains unknown when and where PA is produced in the living cell. Based on the principle of Förster resonance energy transfer (FRET), we generated PA biosensor, and named Pii (phosphatidic acid indicator). In these biosensors, the lipid-binding domain of DOCK2 is sandwiched with the cyan fluorescent protein and yellow fluorescent protein and is tagged with the plasma membrane-targeting sequence of K-Ras. The addition of synthetic PA, or the activation of phospholipase D or diacylglycerol kinase at the plasma membrane, changed the level of FRET in Pii-expressing cells, demonstrating the response of Pii to PA. The biosensor also detected divergent PA content among various cell lines as well as within one cell line. Interestingly, the growth factor-induced increment in PA content correlated negatively with the basal PA content before stimulation, suggesting the presence of an upper threshold in the PA concentration at the plasma membrane. The biosensor also revealed uneven PA distribution within the cell, i.e. the basal level and growth factor-induced accumulation of PA was higher at the cell-free edges than at the cell-cell contact region. An insufficient increase in PA may account for ineffective Ras activation at areas of cell-cell contact. In conclusion, the PA biosensor Pii is a versatile tool for examining heterogeneity in the content and distribution of PA in single cells as well as among different cells.
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