ArticleBiology of reproduction2010
Direct effects of retinoic acid on entry of fetal male germ cells into meiosis in mice.
Article in Biology of reproduction, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- In-vitro spermatogenesis through testis modelling: Toward the generation of testicular organoids.Andrology · 2020Review
- Retinoic acid-stimulated ERK1/2 pathway regulates meiotic initiation in cultured fetal germ cells.PloS one · 2019Article
- Generation of haploid spermatids from chicken embryonal primordial germ cells.International journal of molecular medicine · 2018Article
- Dose-dependent functions of fibroblast growth factor 9 regulate the fate of murine XY primordial germ cells.Biology of reproduction · 2017Article
- Retinoic Acid Is Sufficient for the In Vitro Induction of Mouse Spermatocytes.Stem cell reports · 2016Article
- New discovery of cryptorchidism: Decreased retinoic acid in testicle.Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2016Article
- Loss of MAX results in meiotic entry in mouse embryonic and germline stem cells.Nature communications · 2016Article
- ALDH1A1 provides a source of meiosis-inducing retinoic acid in mouse fetal ovaries.Nature communications · 2016Article
- Male germ-like cell differentiation potential of human umbilical cord Wharton's jelly-derived mesenchymal stem cells in co-culture with human placenta cells in presence of BMP4 and retinoic acid.Iranian journal of basic medical sciences · 2015Article
- Medical school hotline: The Institute for Biogenesis Research: a flower in the Pacific.Hawai'i journal of medicine & public health : a journal of Asia Pacific Medicine & Public Health · 2014Article
- Generation of haploid spermatids with fertilization and development capacity from human spermatogonial stem cells of cryptorchid patients.Stem cell reports · 2014Article
- Cloning and expression characteristics of the pig Stra8 gene.International journal of molecular sciences · 2014Article
- Genetic variants in meiotic program initiation pathway genes are associated with spermatogenic impairment in a Han Chinese population.PloS one · 2013Article
- Transgenic Stra8-EYFP pigs: a model for developing male germ cell technologies.Transgenic research · 2012Article
- Derivation of male germ cells from induced pluripotent stem cells in vitro and in reconstituted seminiferous tubules.Cell proliferation · 2012Article
- Male differentiation of germ cells induced by embryonic age-specific Sertoli cells in mice.Biology of reproduction · 2012Article
- Initiating meiosis: the case for retinoic acid.Biology of reproduction · 2012Review
- A new Speedy/RINGO protein may help regulate male meiosis.Asian journal of andrology · 2011Article
- TOPAZ1, a novel germ cell-specific expressed gene conserved during evolution across vertebrates.PloS one · 2011Article
- Sex determination in mammalian germ cells.Asian journal of andrologyReview
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5 authors at 1 institution in 1 country.
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Abstract
In both male and female germ cells of mice, retinoic acid (RA) is a meiosis-inducing factor. In the present study, we used a germ cell culture system to examine the direct effects of RA on meiotic initiation in male germ cells at the stage when they normally enter mitotic arrest to determine the extent to which fetal male germ cells can respond to exogenous RA to alter their sex-specific pathway. Male germ cells between 13.5 and 15.5 days postcoitum (dpc) were isolated from Pou5fl-green fluorescent protein transgenic fetuses and cultured with or without RA for up to 6 days. In the absence of RA, male germ cells did not undergo DNA replication and did not enter meiosis in culture. However, in the presence of RA, male germ cells isolated at 13.5 dpc expressed Stra8 and initiated the meiotic process. The ratio of cells entering meiosis gradually decreased as cells were isolated progressively at later stages. By 15.5 dpc, isolated male germ cells lost their ability to respond to RA signaling. These cells remained dispersed as single cells and progressed along the male differentiation pathway, as evidenced by the establishment of male-specific methylation imprints regardless of the presence or absence of RA. We conclude that male germ cells maintain sexual bipotency until 14.5 dpc that can be reversed by the addition of RA. Once male germ cells enter mitotic arrest, however, they appear to be committed irreversibly to the male-specific differentiation pathway even in the presence of exogenously added RA.
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