ArticleJournal of structural biology2011
Cryo transmission X-ray imaging of the malaria parasite, P. falciparum.
Article in Journal of structural biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 64 citations in OpenAlex.
- Optimisation of freeze substitution protocols for the examination of malaria parasite structure by volumetric electron microscopy.Journal of microscopy · 2026Article
- Biophysical Tools and Concepts Enable Understanding of Asexual Blood Stage Malaria.Frontiers in cellular and infection microbiology · 2022Review
- Malaria Pigment Crystals: The Achilles' Heel of the Malaria Parasite.ChemMedChem · 2021Review
- Article
- The cellular landscape by cryo soft X-ray tomography.Biophysical reviews · 2019Review
- Multimodal analysis of Plasmodium knowlesi-infected erythrocytes reveals large invaginations, swelling of the host cell, and rheological defects.Cellular microbiology · 2019Article
- Imaging cell morphology and physiology using X-rays.Biochemical Society transactions · 2019Review
- Comparison of 3D cellular imaging techniques based on scanned electron probes: Serial block face SEM vs. Axial bright-field STEM tomography.Journal of structural biology · 2018Article
- Progress in imaging methods: insights gained into Plasmodium biology.Nature reviews. Microbiology · 2017Review
- Three-dimensional imaging of human stem cells using soft X-ray tomography.Journal of the Royal Society, Interface · 2015Article
- Article
- Review
- Whole-cell phase contrast imaging at the nanoscale using Fresnel coherent diffractive imaging tomography.Scientific reports · 2013Article
- Oriented nucleation of hemozoin at the digestive vacuole membrane in Plasmodium falciparum.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Aligned hemozoin crystals in curved clusters in malarial red blood cells revealed by nanoprobe X-ray Fe fluorescence and diffraction.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- A 95 kDa protein of Plasmodium vivax and P. cynomolgi visualized by three-dimensional tomography in the caveola-vesicle complexes (Schüffner's dots) of infected erythrocytes is a member of the PHIST family.Molecular microbiology · 2012Article
- Visualizing and quantifying cell phenotype using soft X-ray tomography.BioEssays : news and reviews in molecular, cellular and developmental biology · 2012Review
- Soft X-ray microscopy analysis of cell volume and hemoglobin content in erythrocytes infected with asexual and sexual stages of Plasmodium falciparum.Journal of structural biology · 2012Article
- Cryo X-ray nano-tomography of vaccinia virus infected cells.Journal of structural biology · 2012Article
- Investigation of the Plasmodium falciparum food vacuole through inducible expression of the chloroquine resistance transporter (PfCRT).PloS one · 2012Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Cryo transmission X-ray microscopy in the "water window" of photon energies has recently been introduced as a method that exploits the natural contrast of biological samples. We have used cryo tomographic X-ray imaging of the intra-erythrocytic malaria parasite, Plasmodium falciparum, to undertake a survey of the cellular features of this important human pathogen. We examined whole hydrated cells at different stages of growth and defined some of the structures with different X-ray density, including the parasite nucleus, cytoplasm, digestive vacuole and the hemoglobin degradation product, hemozoin. As the parasite develops from an early cup-shaped morphology to a more rounded shape, puncta of hemozoin are formed; these coalesce in the mature trophozoite into a central compartment. In some trophozoite stage parasites we observed invaginations of the parasite surface and, using a selective permeabilization process, showed that these remain connected to the RBC cytoplasm. Some of these invaginations have large openings consistent with phagocytic structures and we observed independent endocytic vesicles in the parasite cytoplasm which appear to play a role in hemoglobin uptake. In schizont stage parasites staggered mitosis was observed and X-ray-dense lipid-rich structures were evident at their apical ends of the developing daughter cells. Treatment of parasites with the antimalarial drug artemisinin appears to affect parasite development and their ability to produce the hemoglobin breakdown product, hemozoin.
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