ArticleThe FEBS journal2010
Enzymatic control of anhydrobiosis-related accumulation of trehalose in the sleeping chironomid, Polypedilum vanderplanki.
Article in The FEBS journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed.
- Review
- Effects of Orally Delivered Double-Stranded RNA ofInsects · 2025Article
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- The Role ofInsects · 2024Article
- A sodium-dependent trehalose transporter contributes to anhydrobiosis in insect cell line, Pv11.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Labile assembly of a tardigrade protein induces biostasis.Protein science : a publication of the Protein Society · 2024Article
- Trehalose-6-Phosphate Synthase Contributes to Rapid Cold Hardening in the Invasive InsectInsects · 2023Article
- Article
- Silencing ofInternational journal of molecular sciences · 2022Article
- Article
- High quality genome assembly of the anhydrobiotic midge provides insights on a single chromosome-based emergence of extreme desiccation tolerance.NAR genomics and bioinformatics · 2022Article
- Article
- The Modulation of Trehalose Metabolism by 20-Hydroxyecdysone in Antheraea pernyi (Lepidoptera: Saturniidae) During its Diapause Termination and Post-Termination Period.Journal of insect science (Online) · 2020Article
- Combined metabolome and transcriptome analysis reveals key components of complete desiccation tolerance in an anhydrobiotic insect.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Regulatory Functions ofFrontiers in physiology · 2020Article
- Comparative proteomics of stenotopic caddisfly Crunoecia irrorata identifies acclimation strategies to warming.Molecular ecology · 2019Article
- Transcriptome analysis of the anhydrobiotic cell line Pv11 infers the mechanism of desiccation tolerance and recovery.Scientific reports · 2018Article
- Gene Cloning, Prokaryotic Expression, and Biochemical Characterization of a Soluble Trehalase in Helicoverpa armigera Hübner (Lepidoptera: Noctuidae).Journal of insect science (Online) · 2018Article
- Cooption of heat shock regulatory system for anhydrobiosis in the sleeping chironomidProceedings of the National Academy of Sciences of the United States of America · 2018Article
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10 authors.
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Abstract
Larvae of an anhydrobiotic insect, Polypedilum vanderplanki, accumulate very large amounts of trehalose as a compatible solute on desiccation, but the molecular mechanisms underlying this accumulation are unclear. We therefore isolated the genes coding for trehalose metabolism enzymes, i.e. trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP) for the synthesis step, and trehalase (TREH) for the degradation step. Although computational prediction indicated that the alternative splicing variants (PvTpsα/β) obtained encoded probable functional motifs consisting of a typical consensus domain of TPS and a conserved sequence of TPP, PvTpsα did not exert activity as TPP, but only as TPS. Instead, a distinct gene (PvTpp) obtained expressed TPP activity. Previous reports have suggested that insect TPS is, exceptionally, a bifunctional enzyme governing both TPS and TPP. In this article, we propose that TPS and TPP activities in insects can be attributed to discrete genes. The translated product of the TREH ortholog (PvTreh) certainly degraded trehalose to glucose. Trehalose was synthesized abundantly, consistent with increased activities of TPS and TPP and suppressed TREH activity. These results show that trehalose accumulation observed during anhydrobiosis induction in desiccating larvae can be attributed to the activation of the trehalose synthetic pathway and to the depression of trehalose hydrolysis.
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