ArticleCellular and molecular bioengineering2010
Manipulation of Suspended Single Cells by Microfluidics and Optical Tweezers.
Article in Cellular and molecular bioengineering, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Progress of Microfluidic Continuous Separation Techniques for Micro-/Nanoscale Bioparticles.Biosensors · 2021Review
- Differentiation of single lymphoma primary cells and normal B-cells based on their adhesion to mesenchymal stromal cells in optical tweezers.Scientific reports · 2019Article
- Microfluidic extensional rheometry using stagnation point flow.Biomicrofluidics · 2016Review
- Microfluidic device capable of medium recirculation for non-adherent cell culture.Biomicrofluidics · 2014Article
- Microfluidic platforms for mechanobiology.Lab on a chip · 2013Review
- Measuring cell mechanics by optical alignment compression cytometry.Lab on a chip · 2013Article
- Flow loading induces oscillatory trajectories in a bloodstream parasite.Biophysical journal · 2012Article
- A hyphenated optical trap capillary electrophoresis laser induced native fluorescence system for single-cell chemical analysis.The Analyst · 2012Article
- Cytoskeletal strains in modeled optohydrodynamically stressed healthy and diseased biological cells.Journal of biophysics (Hindawi Publishing Corporation : Online) · 2012Article
- Volumetric stress-strain analysis of optohydrodynamically suspended biological cells.Journal of biomechanical engineering · 2011Article
- Two-Dimensional Modeling of Nanomechanical Strains in Healthy and Diseased Single-Cells During Microfluidic Stress Applications.Journal of nanotechnology in engineering and medicine · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Chondrocytes and osteoblasts experience multiple stresses in vivo. The optimum mechanical conditions for cell health are not fully understood. This paper describes the optical and microfluidic mechanical manipulation of single suspended cells enabled by the μPIVOT, an integrated micron resolution particle image velocimeter (μPIV) and dual optical tweezers instrument (OT). In this study, we examine the viability and trap stiffness of cartilage cells, identify the maximum fluid-induced stresses possible in uniform and extensional flows, and compare the deformation characteristics of bone and muscle cells. These results indicate cell photodamage of chondrocytes is negligible for at least 20 min for laser powers below 30 mW, a dead cell presents less resistance to internal organelle rearrangement and deforms globally more than a viable cell, the maximum fluid-induced shear stresses are limited to ~15 mPa for uniform flows but may exceed 1 Pa for extensional flows, and osteoblasts show no deformation for shear stresses up to 250 mPa while myoblasts are more easily deformed and exhibit a modulated response to increasing stress. This suggests that global and/or local stresses can be applied to single cells without physical contact. Coupled with microfluidic sensors, these manipulations may provide unique methods to explore single cell biomechanics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.