ArticlePLoS pathogens2010
The canine papillomavirus and gamma HPV E7 proteins use an alternative domain to bind and destabilize the retinoblastoma protein.
Article in PLoS pathogens, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 44 citations in OpenAlex.
- Complete genome and phylogenetic characterization of a novel papillomavirus from Cuniculus paca in the Brazilian Amazon.Archives of virology · 2026Article
- MmuPV1 E7 promotes phenotypes associated with "high-risk" HPV infection in mouse keratinocytes.Journal of virology · 2025Article
- Crystal structures of the μ2 subunit of clathrin-adaptor protein 2 in complex with peptides derived from human papillomavirus 16 E7.Journal of microbiology (Seoul, Korea) · 2025Article
- Canine papillomavirus: status of diagnostic methods and vaccine innovations.Virology journal · 2025Review
- A novel papillomavirus in a New Zealand fur seal (Arctocephalus forsteri) with oral lesions.Npj viruses · 2024Article
- Novel Gammapapillomavirus type in the nasal cavity of a wild red colobus (Piliocolobus tephrosceles).Access microbiology · 2024Article
- Human papillomaviruses: Knowns, mysteries, and unchartered territories.Journal of medical virology · 2023Review
- MmuPV1 E7's interaction with PTPN14 delays Epithelial differentiation and contributes to virus-induced skin disease.PLoS pathogens · 2023Article
- Review
- Pathological Similarities in the Development of Papillomavirus-Associated Cancer in Humans, Dogs, and Cats.Animals : an open access journal from MDPI · 2022Review
- Article
- Canine Papillomavirus 2 E6 Does Not Interfere With UVB-Induced Upregulation of p53 and p53-Regulated Genes.Frontiers in veterinary science · 2021Article
- Natural cardenolides suppress coronaviral replication by downregulating JAK1 via a NaBiochemical pharmacology · 2020Article
- Article
- Detection of human papillomaviruses in paired healthy skin and actinic keratosis by next generation sequencing.Papillomavirus research (Amsterdam, Netherlands) · 2020Article
- Papillomavirus DNA not detected in canine lobular orbital adenoma and normal conjunctival tissue.BMC veterinary research · 2019Article
- Viral genome integration of canine papillomavirus 16.Papillomavirus research (Amsterdam, Netherlands) · 2019Article
- Nasal virome of dogs with respiratory infection signs include novel taupapillomaviruses.Virus genes · 2019Article
- Diverse papillomaviruses identified in Weddell seals.The Journal of general virology · 2018Article
- Inhibition of TGF-β and NOTCH Signaling by Cutaneous Papillomaviruses.Frontiers in microbiology · 2018Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The high-risk HPV E6 and E7 proteins cooperate to immortalize primary human cervical cells and the E7 protein can independently transform fibroblasts in vitro, primarily due to its ability to associate with and degrade the retinoblastoma tumor suppressor protein, pRb. The binding of E7 to pRb is mediated by a conserved Leu-X-Cys-X-Glu (LXCXE) motif in the conserved region 2 (CR2) of E7 and this domain is both necessary and sufficient for E7/pRb association. In the current study, we report that the E7 protein of the malignancy-associated canine papillomavirus type 2 encodes an E7 protein that has serine substituted for cysteine in the LXCXE motif. In HPV, this substitution in E7 abrogates pRb binding and degradation. However, despite variation at this critical site, the canine papillomavirus E7 protein still bound and degraded pRb. Even complete deletion of the LXSXE domain of canine E7 failed to interfere with binding to pRb in vitro and in vivo. Rather, the dominant binding site for pRb mapped to the C-terminal domain of canine E7. Finally, while the CR1 and CR2 domains of HPV E7 are sufficient for degradation of pRb, the C-terminal region of canine E7 was also required for pRb degradation. Screening of HPV genome sequences revealed that the LXSXE motif of the canine E7 protein was also present in the gamma HPVs and we demonstrate that the gamma HPV-4 E7 protein also binds pRb in a similar way. It appears, therefore, that the type 2 canine PV and gamma-type HPVs not only share similar properties with respect to tissue specificity and association with immunosuppression, but also the mechanism by which their E7 proteins interact with pRb.
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