ArticleActa crystallographica. Section F, Structural biology and crystallization communications2010
Mammalian cell expression, purification, crystallization and microcrystal data collection of autotaxin/ENPP2, a secreted mammalian glycoprotein.
Article in Acta crystallographica. Section F, Structural biology and crystallization communications, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- Linking medicinal cannabis to autotaxin-lysophosphatidic acid signaling.Life science alliance · 2023Article
- Structure-Based Discovery of Novel Chemical Classes of Autotaxin Inhibitors.International journal of molecular sciences · 2020Article
- Getting the Most Out of Your Crystals: Data Collection at the New High-Flux, Microfocus MX Beamlines at NSLS-II.Molecules (Basel, Switzerland) · 2019Article
- Lysophosphatidic acid produced by autotaxin acts as an allosteric modulator of its catalytic efficiency.The Journal of biological chemistry · 2018Article
- Synaptic phospholipids as a new target for cortical hyperexcitability and E/I balance in psychiatric disorders.Molecular psychiatry · 2018Article
- Current advances in synchrotron radiation instrumentation for MX experiments.Archives of biochemistry and biophysics · 2016Review
- Steroid binding to Autotaxin links bile salts and lysophosphatidic acid signalling.Nature communications · 2016Article
- The polybasic insertion in autotaxin α confers specific binding to heparin and cell surface heparan sulfate proteoglycans.The Journal of biological chemistry · 2013Article
- Crystallization and preliminary X-ray crystallographic analysis of human autotaxin.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011Article
- The design of macromolecular crystallography diffraction experiments.Acta crystallographica. Section D, Biological crystallography · 2011Article
- Autotaxin: its role in biology of melanoma cells and as a pharmacological target.Enzyme research · 2011Article
- Structural basis of substrate discrimination and integrin binding by autotaxin.Nature structural & molecular biology · 2011Article
- Crystallization and preliminary X-ray diffraction analysis of rat autotaxin.Acta crystallographica. Section F, Structural biology and crystallization communications · 2010Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autotaxin (ATX or ENPP2) is a secreted glycosylated mammalian enzyme that exhibits lysophospholipase D activity, hydrolyzing lysophosphatidylcholine to the signalling lipid lysophosphatidic acid. ATX is an approximately 100 kDa multi-domain protein encompassing two N-terminal somatomedin B-like domains, a central catalytic phosphodiesterase domain and a C-terminal nuclease-like domain. Protocols for the efficient expression of ATX from stably transfected mammalian HEK293 cells in amounts sufficient for crystallographic studies are reported. Purification resulted in protein that crystallized readily, but various attempts to grow crystals suitable in size for routine crystallographic structure determination were not successful. However, the available micrometre-thick plates diffracted X-rays beyond 2.0 A resolution and allowed the collection of complete diffraction data to about 2.6 A resolution. The problems encountered and the current advantages and limitations of diffraction data collection from thin crystal plates are discussed.
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