Evidence map›Paper›PMID 20823515›Full record

ArticleActa crystallographica. Section F, Structural biology and crystallization communications2010

Metal-ion dependence of the active-site conformation of the translesion DNA polymerase Dpo4 from Sulfolobus solfataricus.

Adriana Irimia, Lioudmila V Loukachevitch, Robert L Eoff, F Peter Guengerich, Martin Egli

Abstract read
In one paragraph

Article in Acta crystallographica. Section F, Structural biology and crystallization communications, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Translesion DNA Synthesis.EcoSal Plus · 2012
    Article
  10. Article
  11. Article
  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Adriana IrimiaDepartment of Biochemistry and Center in Molecular Toxicology, Vanderbilt University, Nashville, TN 37232, USA.
Lioudmila V Loukachevitch
Robert L Eoff
F Peter Guengerich
Martin Egli

Funding

Structural Biology Facility CoreP30ES000267 · NIEHS · VANDERBILT UNIVERSITY · PI GUENGERICH, F PETER · 1985 to 2014
$20.3M
SYNTHETIC APPROACHES TO PAH-ADDUCTED NUCLEOTIDESP01ES005355 · NIEHS · VANDERBILT UNIVERSITY · PI STONE, MICHAEL P · 1991 to 2010
$13.4M
POLYMERASE INTERACTIONS WITH CARCINOGEN MODIFIED DNAR01ES010375 · NIEHS · VANDERBILT UNIVERSITY · PI GUENGERICH, F PETER · 2000 to 2014
$5.9M
Translesion Synthesis Opposite Carcinogen Bound DNAF32CA119776 · NCI · VANDERBILT UNIVERSITY · PI EOFF, ROBERT L · 2006 to 2008
$142k
Coordinating Translesion DNA Synthesis Opposite Damaged DNAK99GM084460 · NIGMS · VANDERBILT UNIVERSITY · PI EOFF, ROBERT L · 2009 to 2010
$111k
NCI NIH HHS F32 CA119776NIEHS NIH HHS P01 ES005355NIEHS NIH HHS P01 ES05355NIEHS NIH HHS P30 ES000267NIEHS NIH HHS R01 ES010375NIGMS NIH HHS K99 GM084460
6 · The paper itself

Abstract

Crystal structures of a binary Mg2+-form Dpo4-DNA complex with 1,N2-etheno-dG in the template strand as well as of ternary Mg2+-form Dpo4-DNA-dCTP/dGTP complexes with 8-oxoG in the template strand have been determined. Comparison of their conformations and active-site geometries with those of the corresponding Ca2+-form complexes revealed that the DNA and polymerase undergo subtle changes as a result of the catalytically more active Mg2+ occupying both the A and B sites.

Indexed as

Catalytic DomainBiocatalysisCalciumCations, DivalentCrystallography, X-RayDNA Polymerase betaMagnesiumModels, MolecularProtein BindingStructural Homology, ProteinSulfolobus solfataricusCalciumCations, DivalentDNA Polymerase betaMagnesium

Identifiers

PMID20823515
PMCPMC2935216

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.