ArticleProceedings of the National Academy of Sciences of the United States of America2010
Selective cell death mediated by small conditional RNAs.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 88 citations in OpenAlex.
- Triggering RNAi with multifunctional RNA nanoparticles and their delivery.DNA and RNA nanotechnology · 2015Article
- Production of Fatty Acid-derived valuable chemicals in synthetic microbes.Frontiers in bioengineering and biotechnology · 2014Review
- "Selective cell death mediated by small conditional RNAs" is not selective.Cancer biology & therapy · 2013Article
- DNA branch migration reactions through photocontrollable toehold formation.Journal of the American Chemical Society · 2013Article
- Self-assembled, aptamer-tethered DNA nanotrains for targeted transport of molecular drugs in cancer theranostics.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Activation of different split functionalities on re-association of RNA-DNA hybrids.Nature nanotechnology · 2013Article
- Synthetic genomics and synthetic biology applications between hopes and concerns.Current genomics · 2013Article
- Dynamic DNA assemblies mediated by binding-induced DNA strand displacement.Journal of the American Chemical Society · 2013Article
- A logical molecular circuit for programmable and autonomous regulation of protein activity using DNA aptamer-protein interactions.Journal of the American Chemical Society · 2012Article
- Activation of PKR by RNA misfolding: HDV ribozyme dimers activate PKR.RNA (New York, N.Y.) · 2012Article
- De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Multiplexed in situ immunofluorescence using dynamic DNA complexes.Angewandte Chemie (International ed. in English) · 2012Article
- Synthetic in vitro circuits.Current opinion in chemical biology · 2012Review
- Meta-DNA: synthetic biology via DNA nanostructures and hybridization reactions.Journal of the Royal Society, Interface · 2012Article
- Design and analysis of DNA strand displacement devices using probabilistic model checking.Journal of the Royal Society, Interface · 2012Article
- Configuring robust DNA strand displacement reactions for in situ molecular analyses.Nucleic acids research · 2012Article
- Abstractions for DNA circuit design.Journal of the Royal Society, Interface · 2012Article
- Optimizing the specificity of nucleic acid hybridization.Nature chemistry · 2012Article
- A programmable NOR-based device for transcription profile analysis.Scientific reports · 2012Article
- Challenges and opportunities for structural DNA nanotechnology.Nature nanotechnology · 2011Review
Corrections and comments
- Retraction · 2012-12-17Falsification/Fabrication of Data · Investigation by Company/Institution · Investigation by ORI · Misconduct - Official Investigation(s) and/or Finding(s) · Misconduct by Author ·
- Retracted
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Cancer cells are characterized by genetic mutations that deregulate cell proliferation and suppress cell death. To arrest the uncontrolled replication of malignant cells, conventional chemotherapies systemically disrupt cell division, causing diverse and often severe side effects as a result of collateral damage to normal cells. Seeking to address this shortcoming, we pursue therapeutic regulation that is conditional, activating selectively in cancer cells. This functionality is achieved using small conditional RNAs that interact and change conformation to mechanically transduce between detection of a cancer mutation and activation of a therapeutic pathway. Here, we describe small conditional RNAs that undergo hybridization chain reactions (HCR) to induce cell death via an innate immune response if and only if a cognate mRNA cancer marker is detected within a cell. The sequences of the small conditional RNAs can be designed to accept different mRNA markers as inputs to HCR transduction, providing a programmable framework for selective killing of diverse cancer cells. In cultured human cancer cells (glioblastoma, prostate carcinoma, Ewing's sarcoma), HCR transduction mediates cell death with striking efficacy and selectivity, yielding a 20- to 100-fold reduction in population for cells containing a cognate marker, and no measurable reduction otherwise. Our results indicate that programmable mechanical transduction with small conditional RNAs represents a fundamental principle for exploring therapeutic conditional regulation in living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.