ArticleInfection and immunity2010
Mannheimia haemolytica and its leukotoxin cause neutrophil extracellular trap formation by bovine neutrophils.
Article in Infection and immunity, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
51 citing papers in PubMed, 90 citations in OpenAlex.
- Transcriptomic analysis of naturally occurring bovine respiratory disease in Australian feedlot cattle identifies bovine viral diarrhoea virus-induced immunosuppression and Mollicutes as the dominant drivers of lung inflammation.Veterinary research · 2026Article
- Target neutrophil heterogeneity and plasticity in cancer.Journal of hematology & oncology · 2025Review
- Exploring the diagnostic landscape ofFrontiers in microbiology · 2025Review
- Article
- Article
- New Insights into Neutrophil Extracellular Trap (NETs) Formation from Porcine Neutrophils in Response to Bacterial Infections.International journal of molecular sciences · 2022Article
- Review of Associated Health Benefits of Algal Supplementation in Cattle with Reference to Bovine Respiratory Disease Complex in Feedlot Systems.Animals : an open access journal from MDPI · 2022Review
- Chicken heterophils extracellular traps act as early effectors against cyclopiazonic acid dependent upon NADPH oxidase, ROS and glycolysis.Archives of toxicology · 2022Article
- The Expanding Role of Extracellular Traps in Inflammation and Autoimmunity: The New Players in Casting Dark Webs.International journal of molecular sciences · 2022Review
- Bovine respiratory microbiota of feedlot cattle and its association with disease.Veterinary research · 2022Review
- Review
- Role of Cellular Metabolism in the Formation of Neutrophil Extracellular Traps in Airway Diseases.Frontiers in immunology · 2022Review
- Biological and molecular characterization of a sheep pathogen isolate ofVeterinary world · 2021Article
- A Review of the Neutrophil Extracellular Traps (NETs) from Cow, Sheep and Goat Models.International journal of molecular sciences · 2021Review
- Uncovering the Oral Dysbiotic Microbiota as Masters of Neutrophil Responses in the Pathobiology of Periodontitis.Frontiers in microbiology · 2021Review
- The Effects of Ursolic Acid Treatment on Immunopathogenesis FollowingFrontiers in veterinary science · 2021Article
- The Immunology of Bovine Respiratory Disease: Recent Advancements.The Veterinary clinics of North America. Food animal practice · 2020Review
- In vivo evidence for extracellular DNA trap formation.Cell death & disease · 2020Review
- Review
- Neutrophil Extracellular Traps in the Second Decade.Journal of innate immunity · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mannheimia haemolytica is an important member of the bovine respiratory disease complex, which is characterized by abundant neutrophil infiltration into the alveoli and fibrin deposition. Recently several authors have reported that human neutrophils release neutrophil extracellular traps (NETs), which are protein-studded DNA matrices capable of trapping and killing pathogens. Here, we demonstrate that the leukotoxin (LKT) of M. haemolytica causes NET formation by bovine neutrophils in a CD18-dependent manner. Using an unacylated, noncytotoxic pro-LKT produced by an ΔlktC mutant of M. haemolytica, we show that binding of unacylated pro-LKT stimulates NET formation despite a lack of cytotoxicity. Inhibition of LKT binding to the CD18 chain of lymphocyte function-associated antigen 1 (LFA-1) on bovine neutrophils reduced NET formation in response to LKT or M. haemolytica cells. Further investigation revealed that NETs formed in response to M. haemolytica are capable of trapping and killing a portion of the bacterial cells. NET formation was confirmed by confocal microscopy and by scanning and transmission electron microscopy. Prior exposure of bovine neutrophils to LKT enhanced subsequent trapping and killing of M. haemolytica cells in bovine NETs. Understanding NET formation in response to M. haemolytica and its LKT provides a new perspective on how neutrophils contribute to the pathogenesis of bovine respiratory disease.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.