ArticleInfection and immunity2010
Enteroaggregative Escherichia coli disrupts epithelial cell tight junctions.
Article in Infection and immunity, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 104 citations in OpenAlex.
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- The Effect of Bacterial Infections, Probiotics and Zonulin on Intestinal Barrier Integrity.International journal of molecular sciences · 2021Review
- Effect of substrate stiffness on human intestinal enteroids' infectivity by enteroaggregative Escherichia coli.Acta biomaterialia · 2021Article
- Tight Junctions as a Key for Pathogens Invasion in Intestinal Epithelial Cells.International journal of molecular sciences · 2021Review
- Redefining enteroaggregative Escherichia coli (EAEC): Genomic characterization of epidemiological EAEC strains.PLoS neglected tropical diseases · 2020Article
- Aggregative Adherence Fimbriae II of Enteroaggregative Escherichia coli Are Required for Adherence and Barrier Disruption during Infection of Human Colonoids.Infection and immunity · 2020Article
- Surface Protein Dispersin of EnteroaggregativeFrontiers in microbiology · 2020Article
- Investigation of Twenty Metal, Metal Oxide, and Metal Sulfide Nanoparticles' Impact on Differentiated Caco-2 Monolayer Integrity.NanoImpact · 2020Article
- Oxygen and contact with human intestinal epithelium independently stimulate virulence gene expression in enteroaggregative Escherichia coli.Cellular microbiology · 2019Article
- The Role of the AggR Regulon in the Virulence of the Shiga Toxin-Producing EnteroaggregativeFrontiers in microbiology · 2019Article
- TLR4 Participates in the Inflammatory Response Induced by the AAF/II Fimbriae From EnteroaggregativeFrontiers in cellular and infection microbiology · 2019Article
- EnteroaggregativemBio · 2017Article
- Structural and functional studies of Escherichia coli aggregative adherence fimbriae (AAF/V) reveal a deficiency in extracellular matrix binding.Biochimica et biophysica acta. Proteins and proteomics · 2017Article
- Pet serine protease from enteroaggregative Escherichia coli stimulates the inflammatory response activating human macrophages.BMC microbiology · 2016Article
- Shiga toxin 2a and Enteroaggregative Escherichia coli--a deadly combination.Gut microbes · 2015Review
- The presence of the pAA plasmid in the German O104:H4 Shiga toxin type 2a (Stx2a)-producing enteroaggregative Escherichia coli strain promotes the translocation of Stx2a across an epithelial cell monolayer.The Journal of infectious diseases · 2014Article
- The role of epithelial tight junctions involved in pathogen infections.Molecular biology reports · 2014Review
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
Enteroaggregative Escherichia coli (EAEC) is responsible for inflammatory diarrhea in diverse populations, but its mechanisms of pathogenesis have not been fully elucidated. We have used a previously characterized polarized intestinal T84 cell model to investigate the effects of infection with EAEC strain 042 on tight junction integrity. We find that infection with strain 042 induces a decrease in transepithelial electrical resistance (TER) compared to uninfected controls and to cells infected with commensal E. coli strain HS. When the infection was limited after 3 h by washing and application of gentamicin, we observed that the TER of EAEC-infected monolayers continued to decline, and they remained low even as long as 48 h after the infection. Cells infected with the afimbrial mutant strain 042aafA exhibited TER measurements similar to those seen in uninfected monolayers, implicating the aggregative adherence fimbriae II (AAF/II) as necessary for barrier dysfunction. Infection with wild-type strain 042 induced aberrant localization of the tight junction proteins claudin-1 and, to a lesser degree, occludin. EAEC-infected T84 cells exhibited irregular shapes, and some cells became elongated and/or enlarged; these effects were not observed after infection with commensal E. coli strain HS or 042aafA. The effects on tight junctions were also observed with AAF/I-producing strain JM221, and an afimbrial mutant was similarly deficient in inducing barrier dysfunction. Our results show that EAEC induces epithelial barrier dysfunction in vitro and implicates the AAF adhesins in this phenotype.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.