ArticleMolecular & cellular proteomics : MCP2010
The Penicillium chrysogenum extracellular proteome. Conversion from a food-rotting strain to a versatile cell factory for white biotechnology.
Article in Molecular & cellular proteomics : MCP, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 76 citations in OpenAlex.
- Optimisation and Scale-Up of the Heterologous Production of the Antifungal Protein PeAfpA From Penicillium expansum in Stirred-Tank Bioreactors.Microbial biotechnology · 2026Article
- Proteomic profiling of amylases secreted by3 Biotech · 2026Article
- Biochemical characteristics of extracts from proallergenic microfungi Erysiphe Palczewskii and Erysiphe convolvuli.BMC genomics · 2025Article
- Genome sequencing and analysis of penicillin V producing Penicillium rubens strain BIONCL P45 isolated from India.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- Biotechnological Fungal Platforms for the Production of Biosynthetic Cannabinoids.Journal of fungi (Basel, Switzerland) · 2023Review
- Review
- Development of a FungalBraid Penicillium expansum-based expression system for the production of antifungal proteins in fungal biofactories.Microbial biotechnology · 2022Article
- Bacterial Quorum-Quenching Lactonase Hydrolyzes Fungal Mycotoxin and Reduces Pathogenicity ofJournal of fungi (Basel, Switzerland) · 2021Article
- Omics Approaches Applied toGenes · 2020Review
- Insight into the Genome of DiverseInternational journal of molecular sciences · 2020Review
- Molecular Mechanisms behind Free Radical Scavengers Function against Oxidative Stress.Antioxidants (Basel, Switzerland) · 2017Review
- Key role of LaeA and velvet complex proteins on expression of β-lactam and PR-toxin genes in Penicillium chrysogenum: cross-talk regulation of secondary metabolite pathways.Journal of industrial microbiology & biotechnology · 2017Review
- Differential Proteomic Profiles ofFrontiers in microbiology · 2017Article
- Edaravone leads to proteome changes indicative of neuronal cell protection in response to oxidative stress.Neurochemistry international · 2015Article
- Methodologies and perspectives of proteomics applied to filamentous fungi: from sample preparation to secretome analysis.International journal of molecular sciences · 2015Review
- Single-step fermentative production of the cholesterol-lowering drug pravastatin via reprogramming of Penicillium chrysogenum.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Calcium-containing phosphopeptides pave the secretory pathway for efficient protein traffic and secretion in fungi.Microbial cell factories · 2014Review
- Proteome analysis reveals roles of L-DOPA in response to oxidative stress in neurons.BMC neuroscience · 2014Article
- Genomic characteristics and comparative genomics analysis of Penicillium chrysogenum KF-25.BMC genomics · 2014Article
- Alteration of protein patterns in black rock inhabiting fungi as a response to different temperatures.Fungal biology · 2012Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The filamentous fungus Penicillium chrysogenum is well-known by its ability to synthesize β-lactam antibiotics as well as other secondary metabolites. Like other filamentous fungi, this microorganism is an excellent host for secretion of extracellular proteins because of the high capacity of its protein secretion machinery. In this work, we have characterized the extracellular proteome reference map of P. chrysogenum Wisconsin 54-1255 by two-dimensional gel electrophoresis. This method allowed the correct identification of 279 spots by peptide mass fingerprinting and tandem MS. These 279 spots included 328 correctly identified proteins, which corresponded to 131 different proteins and their isoforms. One hundred and two proteins out of 131 were predicted to contain either classical or nonclassical secretion signal peptide sequences, providing evidence of the authentic extracellular location of these proteins. Proteins with higher representation in the extracellular proteome were those involved in plant cell wall degradation (polygalacturonase, pectate lyase, and glucan 1,3-β-glucosidase), utilization of nutrients (extracellular acid phosphatases and 6-hydroxy-d-nicotine oxidase), and stress response (catalase R). This filamentous fungus also secretes enzymes specially relevant for food industry, such as sulfydryl oxidase, dihydroxy-acid dehydratase, or glucoamylase. The identification of several antigens in the extracellular proteome also highlights the importance of this microorganism as one of the main indoor allergens. Comparison of the extracellular proteome among three strains of P. chrysogenum, the wild-type NRRL 1951, the Wis 54-1255 (an improved, moderate penicillin producer), and the AS-P-78 (a penicillin high-producer), provided important insights to consider improved strains of this filamentous fungus as versatile cell-factories of interest, beyond antibiotic production, for other aspects of white biotechnology.
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