ArticleBMC microbiology2010
Global regulation of gene expression in response to cysteine availability in Clostridium perfringens.
Article in BMC microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Genomic and clinical determinants of extraintestinalmedRxiv : the preprint server for health sciences · 2026Article
- Downregulation of the cysE/cysK genes of Clostridioides difficile induced by cysteine revealed the response of bacteria to iron homeostasis.BMC microbiology · 2025Article
- The RgaS-RgaR two-component system promotes Clostridioides difficile sporulation through a small RNA and the Agr1 system.PLoS genetics · 2023Article
- Potent Bile Acid Microbial Metabolites ModulatePathogens (Basel, Switzerland) · 2023Article
- The RgaS-RgaR two-component system promotesbioRxiv : the preprint server for biology · 2023Article
- Induced Production, Synthesis, and Immunomodulatory Action of Clostrisulfone, a Diarylsulfone from Clostridium acetobutylicum.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Transcriptional Profiling of the ProbioticInternational journal of molecular sciences · 2020Article
- Transcriptional Regulation of Cysteine and Methionine Metabolism inFrontiers in microbiology · 2018Article
- Microbial Diversity in Sulfate-Reducing Marine Sediment Enrichment Cultures Associated with Anaerobic Biotransformation of Coastal Stockpiled Phosphogypsum (Sfax, Tunisia).Frontiers in microbiology · 2017Article
- Control of Clostridium difficile Physiopathology in Response to Cysteine Availability.Infection and immunity · 2016Article
- Review
- Exploring Amino Acid Auxotrophy in Bifidobacterium bifidum PRL2010.Frontiers in microbiology · 2015Article
- The unique regulation of iron-sulfur cluster biogenesis in a Gram-positive bacterium.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Structural requirement in Clostridium perfringens collagenase mRNA 5' leader sequence for translational induction through small RNA-mRNA base pairing.Journal of bacteriology · 2013Article
- Characterization of the SigD regulon of C. difficile and its positive control of toxin production through the regulation of tcdR.PloS one · 2013Article
- NIBBS-search for fast and accurate prediction of phenotype-biased metabolic systems.PLoS computational biology · 2012Article
- The key sigma factor of transition phase, SigH, controls sporulation, metabolism, and virulence factor expression in Clostridium difficile.Journal of bacteriology · 2011Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCysteine has a crucial role in cellular physiology and its synthesis is tightly controlled due to its reactivity. However, little is known about the sulfur metabolism and its regulation in clostridia compared with other firmicutes. In Clostridium perfringens, the two-component system, VirR/VirS, controls the expression of the ubiG operon involved in methionine to cysteine conversion in addition to the expression of several toxin genes. The existence of links between the C. perfringens virulence regulon and sulfur metabolism prompted us to analyze this metabolism in more detail.
resultsWe first performed a tentative reconstruction of sulfur metabolism in C. perfringens and correlated these data with the growth of strain 13 in the presence of various sulfur sources. Surprisingly, C. perfringens can convert cysteine to methionine by an atypical still uncharacterized pathway. We further compared the expression profiles of strain 13 after growth in the presence of cystine or homocysteine that corresponds to conditions of cysteine depletion. Among the 177 genes differentially expressed, we found genes involved in sulfur metabolism and controlled by premature termination of transcription via a cysteine specific T-box system (cysK-cysE, cysP1 and cysP2) or an S-box riboswitch (metK and metT). We also showed that the ubiG operon was submitted to a triple regulation by cysteine availability via a T-box system, by the VirR/VirS system via the VR-RNA and by the VirX regulatory RNA.In addition, we found that expression of pfoA (theta-toxin), nagL (one of the five genes encoding hyaluronidases) and genes involved in the maintenance of cell redox status was differentially expressed in response to cysteine availability. Finally, we showed that the expression of genes involved in [Fe-S] clusters biogenesis and of the ldh gene encoding the lactate dehydrogenase was induced during cysteine limitation.
conclusionSeveral key functions for the cellular physiology of this anaerobic bacterium were controlled in response to cysteine availability. While most of the genes involved in sulfur metabolism are regulated by premature termination of transcription, other still uncharacterized mechanisms of regulation participated in the induction of gene expression during cysteine starvation.
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